Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and en...

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Bibliographic Details
Published in:Nature biotechnology 1998-07, Vol.16 (7), p.642-646
Main Authors: Cibelli, J.B, Stice, S.L, Golueke, P.J, Kane, J.J, Jerry, J, Blackwell, C, Ponce de Leon, F.A, Robl, J.M
Format: Article
Language:English
Subjects:
ANIMAL TRANSGENIQUE
ANIMALES TRANSGENICOS
Animals
Animals, Genetically Modified
beta-Galactosidase - analysis
Biological and medical sciences
Biotechnology
Blastocyst - physiology
BOVIN
CATTLE
Cattle - genetics
Cell Differentiation
Cell Fusion
CHIMAERAS
Chimera
CHIMERE
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
Ectoderm - physiology
Embryo Transfer
EMBRYONIC DEVELOPMENT
EMBRYONIC STEM CELLS
Endoderm - physiology
Fundamental and applied biological sciences. Psychology
GANADO BOVINO
Gene Transfer Techniques
Genetic engineering
Genetic technics
Genetic Therapy
Mesoderm - physiology
Methods. Procedures. Technologies
NOYAU CELLULAIRE
NUCLEI
NUCLEO
NUCLEUS
QUIMERA
Stem Cells - physiology
TRANSGENIC ANIMALS
Transgenic animals and transgenic plants
TRANSPLANTATION
TRASPLANTES
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Summary:We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt0798-642