Aspirin inhibition and acetylation of the plant cytochrome P450, allene oxide synthase, resembles that of animal prostaglandin endoperoxide H synthase

• Published in: The Journal of biological chemistry 1998-07, Vol.273 (29), p.18139-18145
• Main Authors: Pan, Z. (University of California, Davis), Camara, B, Gardner, H.W, Backhaus, R.A
• Format: Article
• Language: English
• Subjects: ACETILACION; ACETYLATION; ACIDE SALICYLIQUE; ACIDO SALICILICO; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADN; Amino Acid Sequence; AMINO ACID SEQUENCES; Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; ANTIINFLAMMATOIRE; ANTIINFLAMMATORY AGENTS; ANTINFLAMATORIOS; ARABIDOPSIS THALIANA; Aspirin - pharmacology; CAPSICUM ANNUUM; CHEMICAL COMPOSITION; Chromatography, High Pressure Liquid; CITOCROMO P450; COMPLEMENTARY DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Consensus Sequence; Cyclooxygenase Inhibitors - pharmacology; CYTOCHROME P450; DNA; ENZYMIC ACTIVITY; Escherichia coli; GENBANK/1272340 [SIC]; GENBANK/1890152 [SIC]; GENBANK/A56377; GENBANK/P48417; IBUPROFEN; INDOMETACIN; INDOMETACINA; INDOMETHACIN; INDOMETHACINE; INHIBICION; INHIBITION; Intramolecular Oxidoreductases - antagonists & inhibitors; Intramolecular Oxidoreductases - metabolism; Kinetics; LINUM USITATISSIMUM; Models, Chemical; MOLECULAR SEQUENCE DATA; NON-STEROIDAL ANTIINFLAMMATORY AGENTS; NUCLEOTIDE SEQUENCE; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; PARTHENIUM ARGENTATUM; PIROXICAM; Prostaglandin-Endoperoxide Synthases - metabolism; SALICILATOS; SALICYLATE; SALICYLATES; SALICYLIC ACIDS; SECUENCIA NUCLEOTIDICA; Sequence Alignment; SEQUENCE NUCLEOTIDIQUE; UNSPECIFIC MONOOXYGENASE
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.273.29.18139

The enzymatic reactions leading to octadecanoid lipid signaling intermediates in plants are similar to those of animals and are inhibited by nonsteroidal anti-inflammatory drugs (NSAIDs) such as salicylic acid and aspirin. In animals, NSAIDs inhibit the cyclooxygenase (COX) activity of prostaglandin endoperoxide H synthase, which ultimately blocks the formation of prostaglandins. In plants, NSAIDs block the formation of 12-oxo-phytodienoic acid and jasmonates, which are the equivalent signaling compounds. In this study we show that NSAIDs act as competitive inhibitors of allene oxide synthase (AOS), the cytochrome P450 that initiates plant oxylipin synthesis. We also show that aspirin causes the time-dependent inhibition and acetylation of AOS, which leads the irreversible inactivation of this enzyme. This inhibition and acetylation superficially resembles that observed for the inactivation of COX in animals. In AOS, aspirin acetylates three serine residues near the C-terminal region that appear to be highly conserved among AOS sequences from other plants but are not conserved among “classical” type P450s. The role of these serine residues is unclear. Unlike animal COX, where acetylation of a single serine residue within the substrate channel leads to inactivation of prostaglandin endoperoxide H synthase, the three serine residues in AOS are not thought to line the putative substrate channel. Thus, inhibition by aspirin may be by a different mechanism. It is possible that aspirin and related NSAIDs could inhibit other P450s that have motifs similar to AOS and consequently serve as potential biochemical targets for this class of drugs.