Evaluation of neutrophilic function (chemotaxis, phagocytosis and microbicidal activity) in healthy dogs and in dogs suffering from recurrent deep pyoderma

• Published in: Veterinary immunology and immunopathology 1998-07, Vol.64 (2), p.123-131
• Main Authors: Chammas, Patricia P.C, Hagiwara, Mitika K
• Format: Article
• Language: English
• Subjects: Animals; Blood Bactericidal Activity; Chemotaxis; Chemotaxis, Leukocyte; Deep pyoderma; Dog Diseases - immunology; Dogs; Dogs - immunology; Escherichia coli - immunology; Female; In Vitro Techniques; Lipopolysaccharides - pharmacology; Male; Neutrophils - immunology; Phagocytosis; Polymorphonuclear cells; Pyoderma - immunology; Pyoderma - veterinary; Reference Values; Staphylococcus - immunology
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/S0165-2427(98)00130-5

The modified Boyden's technique of chemotaxis (`leading front' method) and the bacterial killing test with Staphylococcus sp. stained with acridine orange were performed with suspensions of granulocytes from 50 healthy dogs. Lipopolisacharide of Escherichia coli, in normal dog serum was used as the chemotactic factor. The mean value for the chemotactic differential found was 30.41±12.14 μ. The optimal concentration of bacteria and opsonins (normal dog serum) was 4 and 15%, respectively, and the ideal incubation period was 30 min. The mean values obtained for phagocytosis percentage, number of bacteria per PMN, phagocytosis index and percentage of dead bacteria, were 97.01±3.22%, 22.20±4.6, 21.53±4.50 and 45.30±9.18%, respectively. Granulocyte functions in 18 dogs with recurrent, chronic, deep pyoderma were assessed by the same methods. No significant difference between the two groups, for any of the evaluated parameters, was found. For elucidation of the pathogenesis of recurrent pyoderma, further studies involving humoral and cellular immunity and the complement system are required.