Localized Avidin/Biotin Derivatization of Glassy Carbon Electrodes Using SECM

Different forms of the microreagent mode of SECM were used to attach biotin or make “clean” spots on micron-sized regions on the surface of a carbon electrode. In the direct-write mode, the SECM probe tip is used as an electrochemical “pen” depositing biotin in micron-sized lines on the carbon subst...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1998-07, Vol.70 (13), p.2601-2606
Main Authors: Nowall, Wilbur B, Wipf, David O, Kuhr, Werner G
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - chemistry
Avidin - analogs & derivatives
Avidin - chemistry
Biological and medical sciences
Biosensing Techniques
Biotechnology
Biotin - chemistry
Carbon
Carbon - chemistry
Chemistry
Electrodes
Enzymes, Immobilized
Fluorescein-5-isothiocyanate - analogs & derivatives
Fluorescein-5-isothiocyanate - chemistry
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
Microscopy, Electron, Scanning - instrumentation
Substrate Specificity
Surface Properties
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Summary:Different forms of the microreagent mode of SECM were used to attach biotin or make “clean” spots on micron-sized regions on the surface of a carbon electrode. In the direct-write mode, the SECM probe tip is used as an electrochemical “pen” depositing biotin in micron-sized lines on the carbon substrate as it is scanned across its surface. In the negative microreagent mode, the SECM probe tip is used as an electrochemical “eraser” cleaning off the surface attached molecules and leaving clean spots on the surface of a globally derivatized carbon surface. This type of simple micromodification of the surface of a carbon electrode will allow the fabrication of biosensors that can potentially be tailor-made for a variety of applications.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac971310b