Invasion-Related Antigens of Campylobacter jejuni

kgA HEp-2 cell culture model was used to investigate the antigens required for epithelial cell penetration by Campylobacter jejuni. Penetration of HEp-2 epithelial cells by C. jejuni was significantly inhibited (P < .05) with C. jejuni lysate and a monoclonal antibody (MAb 1B4) in competitive inh...

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Bibliographic Details
Published in:The Journal of infectious diseases 1990-10, Vol.162 (4), p.888-895
Main Authors: Konkel, Michael E., Babakhani, Farah, Joens, Lynn A.
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Antigens, Bacterial - analysis
Bacteria
Bacterial Adhesion
Biological and medical sciences
Blotting, Western
Campylobacter
Campylobacter fetus - immunology
Campylobacter fetus - pathogenicity
Campylobacter fetus - ultrastructure
Campylobacter jejuni
Electrophoresis, Polyacrylamide Gel
Epithelial cells
Flagella
General aspects
Humans
Immunoblotting
Immunohistochemistry
Infections
Infectious diseases
Laryngeal Neoplasms
Major Articles
Medical sciences
Microscopy, Electron
Monoclonal antibodies
Mucosa
Tumor Cells, Cultured
Virulence
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Summary:kgA HEp-2 cell culture model was used to investigate the antigens required for epithelial cell penetration by Campylobacter jejuni. Penetration of HEp-2 epithelial cells by C. jejuni was significantly inhibited (P < .05) with C. jejuni lysate and a monoclonal antibody (MAb 1B4) in competitive inhibition assays. Immunogold electron microscopy revealed that MAb 1B4 bound to the flagella and cell surface of low-passage (invasive) C. jejuni M 96, whereas only the flagella of high-passage (noninvasive) C. jejuni M 96 were labeled. Western blot analysis revealed that MAb 1B4 identified an epitope on antigens of 64–44 kDa in lysates prepared from invasive and noninvasive isolates. In addition, antigens of 42–38 kDa were recognized in lysates prepared from only invasive C. jejuni strains. Proteinase K and sodium meta-periodate chemical treatment of C. jejuni M 96 lysate changed the mobility of antigens recognized by MAb 1B4. The increase in mobility demonstrated a decrease in size of molecules and suggested that antigens required for HEp-2 cell invasion by Campylobacter species may be glycoprotein in nature.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/162.4.888