Enumerating low densities of genetically engineered Erwinia carotovora in soil

An inexpensive, quantitative, and sensitive technique was developed for detection of genetically engineered Erwinia carotovora in soil samples. Enrichment media, antibiotic resistance, and most probable number (MPN) analysis were used to enumerate as few as 1 to 10 target cells/10 g soil. The MPN te...

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Bibliographic Details
Published in:Letters in applied microbiology 1990-02, Vol.10 (2), p.55-59
Main Authors: Scanferlato, V. S., Orvos, D. R., Lacy, G. H., Jr, J. Cairns
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Colony Count, Microbial
Culture Media
Erwinia - genetics
Erwinia - growth & development
Fundamental and applied biological sciences. Psychology
Genetic Engineering
media (enrichment)
Microbiology
Miscellaneous
Mission oriented research
most probable number
soil
Soil Microbiology
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Summary:An inexpensive, quantitative, and sensitive technique was developed for detection of genetically engineered Erwinia carotovora in soil samples. Enrichment media, antibiotic resistance, and most probable number (MPN) analysis were used to enumerate as few as 1 to 10 target cells/10 g soil. The MPN technique recovered significantly higher cell densities than plating; however, densities estimated by the two techniques were strongly correlated. After inoculation of soil microcosms with genetically engineered E. carotovora, a decline rate of 1.2 log units/g soil/10 days and then subsequent disappearance was observed using the MPN technique.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1990.tb00264.x