Effects of nitric oxide from exogenous nitric oxide donors on osteoblastic metabolism

We examined the effects of nitric oxide (NO) on the differentiation and mineralization of newborn rat calvarial osteoblastic cells (ROB cells) using exogenous NO donors, sodium nitroprusside, 3-(2-hydroxy-1-methyl-2-nitrosohydrazino)- N-methyl-1-propanamine (NOC-7) and 2,2′-(hydroxynitrosohydrazino)...

Full description

Saved in:
Bibliographic Details
Published in:European journal of pharmacology 1998-05, Vol.349 (2), p.345-350
Main Authors: Otsuka, Eri, Hirano, Kiyoko, Matsushita, Shouji, Inoue, Atsuto, Shigehisa Hirose, Yamaguchi, Akira, Hagiwara, Hiromi
Format: Article
Language:English
Subjects:
Animals
Animals, Suckling
Biological and medical sciences
Cell Differentiation - drug effects
Cell physiology
Cells, Cultured
cGMP
Cyclic GMP - biosynthesis
Fundamental and applied biological sciences. Psychology
Mineralization, calcification
Molecular and cellular biology
Nitric oxide (NO)
Nitric oxide (NO) donor
Nitric Oxide - pharmacology
Nitroprusside - pharmacology
Nitroso Compounds - pharmacology
Osteoblast
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - metabolism
Rats
Rats, Wistar
Soluble guanylate cyclase
Space life sciences
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We examined the effects of nitric oxide (NO) on the differentiation and mineralization of newborn rat calvarial osteoblastic cells (ROB cells) using exogenous NO donors, sodium nitroprusside, 3-(2-hydroxy-1-methyl-2-nitrosohydrazino)- N-methyl-1-propanamine (NOC-7) and 2,2′-(hydroxynitrosohydrazino)bis-ethanamine (NOC-18). Sodium nitroprusside and NOC-7 dose-dependently enhanced the rate of production of intracellular cGMP in ROB cells and the rat clonal osteogenic cell line ROB-C26. We used NOC (NOC is the trade name for NO complex manufactured by Dojindo, Kumamoto, Japan) as an NO donor in our experiments because sodium nitroprusside exhibited a marked cytotoxicity. Northern blot analysis revealed that the level of mRNA for osteocalcin, one of the osteoblastic differentiation markers, was enhanced in the ROB cells, which was continuously treated by NOC-18. NOC-18, however, did not affect the level of mRNA for alkaline phosphatase and the activity of alkaline phosphatase. Both the number and the total area of mineralized nodules that are a model of in vitro bone formation were shown to be increased by 10 −5 M NOC-18. Our data suggest that NO might act as a local regulator of the metabolism of osteoblastic cells.
ISSN:0014-2999
1879-0712
DOI:10.1016/S0014-2999(98)00190-3