Acetylcholine-induced, calcium-dependent norepinephrine outflow from peripheral human lymphocytes

Catecholamines (CA) were studied in peripheral human lymphocytes, as well as in the supernatants, after incubation with l-tyrosine and l-dihydroxyphenylalanine ( l-Dopa) for 1 h. The effect that the addition of acetylcholine (ACh), Veratridine, Ionomycin or KCl had on the outflow of norepinephrine (...

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Bibliographic Details
Published in:Journal of neuroimmunology 1998-07, Vol.87 (1), p.82-87
Main Authors: Musso, Natale R, Brenci, Sabrina, Indiveri, Francesco, Lotti, Gaetano
Format: Article
Language:English
Subjects:
Acetylcholine
Acetylcholine - pharmacology
Adult
Calcium
Calcium - physiology
Calcium Channel Blockers - pharmacology
D600
Dose-Response Relationship, Drug
Female
Gallopamil - pharmacology
Humans
Ionomycin
Ionomycin - pharmacology
Levodopa - pharmacology
Lymphocytes
Lymphocytes - drug effects
Lymphocytes - metabolism
Male
Norepinephrine
Norepinephrine - metabolism
Tyrosine - pharmacology
Veratridine
Veratridine - pharmacology
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Summary:Catecholamines (CA) were studied in peripheral human lymphocytes, as well as in the supernatants, after incubation with l-tyrosine and l-dihydroxyphenylalanine ( l-Dopa) for 1 h. The effect that the addition of acetylcholine (ACh), Veratridine, Ionomycin or KCl had on the outflow of norepinephrine (NE) from lymphocytes was also studied. The effect of the addition of methoxyverapamil (D600, a Ca 2+ channel blocker) and cholinergic antagonists had on the ACh-induced NE outflow was assessed. CA were determined by HPLC–ECD, both in the supernatant and in the cell lysates. l-Tyrosine and l-Dopa significantly ( P
ISSN:0165-5728
1872-8421
DOI:10.1016/S0165-5728(98)00057-5