Differential activity of P‐glycoprotein in normal blood lymphocyte subsets

To better understand the phenomenon of P‐glycoprotein (P‐170) expression we investigated lymphocyte subpopulations for P‐170 function in healthy volunteers. Studies were based on three‐colour flow cytometry including the fluorescent probe rhodamine 123 (Rh123), which is transported by P‐170. Marked...

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Bibliographic Details
Published in:British journal of haematology 1998-06, Vol.101 (4), p.722-727
Main Authors: Ludescher, Christof, Pall, Georg, Irschick, Eveline U., Gastl, Günther
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - metabolism
Antigens, CD - metabolism
Antineoplastic agents
ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism
Biological and medical sciences
Drug Resistance, Multiple
flow cytometry
Flow Cytometry - methods
Fluorescent Dyes
General aspects
Hematology
Humans
Killer Cells, Natural - metabolism
lymphocytes
Medical sciences
multidrug resistance
Pharmacology. Drug treatments
P‐glycoprotein
Rhodamine 123
Rhodamines
T-Lymphocyte Subsets - metabolism
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Summary:To better understand the phenomenon of P‐glycoprotein (P‐170) expression we investigated lymphocyte subpopulations for P‐170 function in healthy volunteers. Studies were based on three‐colour flow cytometry including the fluorescent probe rhodamine 123 (Rh123), which is transported by P‐170. Marked Rh123 efflux was detected in CD8+ T lymphocytes with CD8+/CD45RA+ T cells (naive cells) showing significantly higher P‐170 activity as compared with CD8+/CD45RA− cells (P 
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1998.00751.x