Different behavior of two Trypanosoma cruzi major clones : Transmission and circulation in young Bolivian patients

Specificity of two widespread Trypanosoma cruzi clonal genotypes or "clonets" (20 and 39) was first analyzed by hybridization with a large set of T. cruzi stocks characterized by multigenic study relying on both MLEE and RAPD. Then, these clonets were detected in the blood of Chagasic chil...

Full description

Saved in:
Bibliographic Details
Published in:Experimental parasitology 1998-07, Vol.89 (3), p.285-295
Main Authors: BRENIERE, S. F, BOSSENO, M.-F, TELLERIA, J, BASTRENTA, B, YAESIK, N, NOIREAU, F, ALCAZAR, J.-L, BARNABE, C, WINCKER, P, TIBAYRENC, M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bolivia
Chagas Disease - parasitology
Chagas Disease - transmission
Child
Child, Preschool
Cloning, Molecular
DNA Probes - standards
DNA, Kinetoplast - analysis
Female
Genetic Variation
Human protozoal diseases
Humans
Infectious diseases
Insect Vectors - parasitology
Male
Medical sciences
Nucleic Acid Hybridization
Parasitemia - parasitology
Parasitemia - transmission
Parasitic diseases
Phylogeny
Polymerase Chain Reaction
Protozoal diseases
Random Amplified Polymorphic DNA Technique
Sensitivity and Specificity
Time Factors
Triatoma - parasitology
Triatoma infestans
Trypanosoma cruzi
Trypanosoma cruzi - classification
Trypanosoma cruzi - genetics
Trypanosoma cruzi - physiology
Trypanosomiasis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Specificity of two widespread Trypanosoma cruzi clonal genotypes or "clonets" (20 and 39) was first analyzed by hybridization with a large set of T. cruzi stocks characterized by multigenic study relying on both MLEE and RAPD. Then, these clonets were detected in the blood of Chagasic children from a Bolivian endemic area by a combination of polymerase chain reaction and clonet-specific DNA hybridization. The distribution of these clonets in patients was significantly different from that observed in the vectors of the same area (Triatoma infestans). In vectors, clonets 20 and 39 are found with comparable frequencies (0.69 and 0.67, respectively) in contrast with patients, in whom clonet 20 and mixed infections exhibit low frequencies. The Chagasic population can be divided into acute infections and latent infections above the accepted criterion of parasitemia (direct microscopic examination). The results suggest a limited selection in the transmission of the two clonets and a further drastic control of clonet 20 parasitemia by the immune system of children patients.
ISSN:0014-4894
1090-2449
DOI:10.1006/expr.1998.4295