Kinetic study on the degradation of prazepam in acidic aqueous solutions by high-performance liquid chromatography and fourth-order derivative ultraviolet spectrophotometry

• Published in: Journal of pharmaceutical and biomedical analysis 1998-08, Vol.17 (4), p.739-750
• Main Authors: Archontaki, H.A., Panderi, I.E., Gikas, E.E., Parissi-Poulou, M.
• Format: Article
• Language: English
• Subjects: Acidic hydrolysis products; Analysis; Anti-Anxiety Agents - chemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Derivative spectrophotometry; General pharmacology; High-performance liquid chromatography; Hydrogen-Ion Concentration; Kinetic study; Kinetics; Medical sciences; Molecular Structure; Pharmacology. Drug treatments; Prazepam; Prazepam - chemistry; Reproducibility of Results; Solutions; Spectrophotometry, Ultraviolet - methods
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(97)00246-X

A reversed-phase HPLC method was developed for the kinetic investigation of the acidic hydrolysis of prazepam which was carried out in hydrochloric acid solutions of 0.01, 0.1 and 1.0 M. In addition, a fourth-order derivative method for monitoring the parent compound itself was proposed and evaluated. One intermediate was observed by HPLC, which should be formed from breakage of the azomethine linkage. Further slow hydrolysis of the amide bond led to the benzophenone product that was isolated and identified. The mechanism of hydrolysis was biphasic, showing a consecutive reaction with a reversible step. Relative standard deviation was less than 2% for HPLC and less than 5% for the derivative method. Detection limits were 1.2×10 −7 M for the former method and 6.7×10 −7 M for the latter. Accelerated studies at higher temperatures were employed. Results of HPLC and fourth-order derivative methods were statistically the same.