Plasma cell membrane glycoprotein PC-1. cDNA cloning of the human molecule, amino acid sequence, and chromosomal location

The murine cell membrane glycoprotein PC-1 is a homodimer with restricted tissue distribution, being first characterized in plasma cells. We now describe the isolation of cDNA clones encoding the human homolog of the murine PC-1 protein, its complete amino acid sequence, and its chromosomal location...

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Bibliographic Details
Published in:The Journal of biological chemistry 1990-10, Vol.265 (29), p.17506-17511
Main Authors: Buckley, M F, Loveland, K A, McKinstry, W J, Garson, O M, Goding, J W
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blotting, Southern
chromosome 6
Chromosome Mapping
Chromosomes, Human, Pair 6
Cloning, Molecular
DNA - genetics
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Humans
Liver - metabolism
Membrane Glycoproteins - genetics
Mice
Mice, Inbred BALB C
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Phosphoric Diester Hydrolases
Pyrophosphatases
Restriction Mapping
RNA, Messenger - genetics
Sequence Homology, Nucleic Acid
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Summary:The murine cell membrane glycoprotein PC-1 is a homodimer with restricted tissue distribution, being first characterized in plasma cells. We now describe the isolation of cDNA clones encoding the human homolog of the murine PC-1 protein, its complete amino acid sequence, and its chromosomal location. Overall, the amino acid sequence of the human protein is about 80% identical to the murine protein, although the extent of homology varies in different domains. It had not been possible to assign a definitive amino terminus to the murine protein. Comparison of the murine and human sequence necessitates reassignment of the amino terminus, resulting in a cytoplasmic tail of 24 amino acids rather than 58 amino acids as previously published for the mouse. The sequence of several independently obtained cDNA clones indicates that the 3' end of the mRNA is subject to alternative splicing. Southern blots suggest a single copy gene. In situ chromosomal hybridization localizes the gene for human PC-1 to chromosome 6q22-q23, a common site for deletions in human lymphoid neoplasia.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)38193-6