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Photoreceptor coupling in turtle retina
Photoreceptors in the isolated turtle retina of two species of turtle, Chelydra serpentina and Pseudemus scripta elegans, were penetrated with double-barrel electrodes. Physiological responses were recorded through one barrel and Neurobiotin tracer was injected from the other. Intracellular injectio...
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| Published in: | Visual neuroscience 1998-07, Vol.15 (4), p.755-764 |
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| Language: | English |
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| container_title | Visual neuroscience |
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| creator | FIRSOV, M.L. GREEN, D.G. |
| description | Photoreceptors in the isolated turtle retina of two
species of turtle, Chelydra serpentina and
Pseudemus scripta elegans, were penetrated with double-barrel
electrodes. Physiological responses were recorded through
one barrel and Neurobiotin tracer was injected from the
other. Intracellular injection of Neurobiotin revealed
patterns of tracer-coupled photoreceptors. Both the patterns
of tracer coupling and the electrophysiology suggest a
high degree of specificity of connections. Rods seem to
be coupled only to rods and green and red cones seem to
be coupled to cones of the same spectral type. Receptive-field
profiles, measured with a thin, sharply focused slit of
light, often had well-defined peaks and troughs in sensitivity.
We have taken advantage of this observation and used the
position of a peak in sensitivity to locate the position
on the retina of a coupled cell. In one rod, it was possible
to correlate physiological and morphological data and to
show that the peaks in the physiological receptive field
occurred at positions on the retina where there were dye-coupled
cells. This provides direct evidence that gap junctions
produce the physiological coupling between rods. |
| doi_str_mv | 10.1017/S0952523898154147 |
| format | article |
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species of turtle, Chelydra serpentina and
Pseudemus scripta elegans, were penetrated with double-barrel
electrodes. Physiological responses were recorded through
one barrel and Neurobiotin tracer was injected from the
other. Intracellular injection of Neurobiotin revealed
patterns of tracer-coupled photoreceptors. Both the patterns
of tracer coupling and the electrophysiology suggest a
high degree of specificity of connections. Rods seem to
be coupled only to rods and green and red cones seem to
be coupled to cones of the same spectral type. Receptive-field
profiles, measured with a thin, sharply focused slit of
light, often had well-defined peaks and troughs in sensitivity.
We have taken advantage of this observation and used the
position of a peak in sensitivity to locate the position
on the retina of a coupled cell. In one rod, it was possible
to correlate physiological and morphological data and to
show that the peaks in the physiological receptive field
occurred at positions on the retina where there were dye-coupled
cells. This provides direct evidence that gap junctions
produce the physiological coupling between rods.</description><identifier>ISSN: 0952-5238</identifier><identifier>EISSN: 1469-8714</identifier><identifier>DOI: 10.1017/S0952523898154147</identifier><identifier>PMID: 9682876</identifier><language>eng</language><publisher>New York, NY: Cambridge University Press</publisher><subject>Animals ; Biological and medical sciences ; Biotin - analogs & derivatives ; Cell coupling ; Cone ; Eye and associated structures. Visual pathways and centers. Vision ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Gap Junctions - physiology ; Isoquinolines ; Neurobiotin ; Neurons - cytology ; Neurons - physiology ; Retina - anatomy & histology ; Retina - physiology ; Retinal Cone Photoreceptor Cells - cytology ; Retinal Cone Photoreceptor Cells - physiology ; Retinal Rod Photoreceptor Cells - cytology ; Retinal Rod Photoreceptor Cells - physiology ; Rod ; Telodendria ; Turtles ; Vertebrates: nervous system and sense organs ; Visual Pathways</subject><ispartof>Visual neuroscience, 1998-07, Vol.15 (4), p.755-764</ispartof><rights>1998 Cambridge University Press</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-8d63f06a35f1a4165c278d173d0aa53c1944e8980a0bca9843d96952633270b43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0952523898154147/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,72703</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2324279$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9682876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FIRSOV, M.L.</creatorcontrib><creatorcontrib>GREEN, D.G.</creatorcontrib><title>Photoreceptor coupling in turtle retina</title><title>Visual neuroscience</title><addtitle>Vis Neurosci</addtitle><description>Photoreceptors in the isolated turtle retina of two
species of turtle, Chelydra serpentina and
Pseudemus scripta elegans, were penetrated with double-barrel
electrodes. Physiological responses were recorded through
one barrel and Neurobiotin tracer was injected from the
other. Intracellular injection of Neurobiotin revealed
patterns of tracer-coupled photoreceptors. Both the patterns
of tracer coupling and the electrophysiology suggest a
high degree of specificity of connections. Rods seem to
be coupled only to rods and green and red cones seem to
be coupled to cones of the same spectral type. Receptive-field
profiles, measured with a thin, sharply focused slit of
light, often had well-defined peaks and troughs in sensitivity.
We have taken advantage of this observation and used the
position of a peak in sensitivity to locate the position
on the retina of a coupled cell. In one rod, it was possible
to correlate physiological and morphological data and to
show that the peaks in the physiological receptive field
occurred at positions on the retina where there were dye-coupled
cells. This provides direct evidence that gap junctions
produce the physiological coupling between rods.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotin - analogs & derivatives</subject><subject>Cell coupling</subject><subject>Cone</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gap Junctions - physiology</subject><subject>Isoquinolines</subject><subject>Neurobiotin</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>Retina - anatomy & histology</subject><subject>Retina - physiology</subject><subject>Retinal Cone Photoreceptor Cells - cytology</subject><subject>Retinal Cone Photoreceptor Cells - physiology</subject><subject>Retinal Rod Photoreceptor Cells - cytology</subject><subject>Retinal Rod Photoreceptor Cells - physiology</subject><subject>Rod</subject><subject>Telodendria</subject><subject>Turtles</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Visual Pathways</subject><issn>0952-5238</issn><issn>1469-8714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EKqXwASyQskCwCvgV21lCBS2oPAtry3Gc4pImwU4k-HtcNeoGic3M4pwZXV0AjhG8QBDxyzlME5xgIlKBEooo3wFDRFkaC47oLhiucbzm--DA-yWEiKCEDMAgZQILzobg_PmjbmtntGnCinTdNaWtFpGtorZzbWkiZ1pbqUOwV6jSm6N-j8D77c3beBrPniZ346tZrCnkbSxyRgrIFEkKpChiicZc5IiTHCqVEI1SSk1ICxXMtEoFJXnKQkhGCOYwo2QEzjZ_G1d_dca3cmW9NmWpKlN3XgoICYFhjADaiNrV3jtTyMbZlXI_EkG5Lkf-KSfcnPTPu2xl8u1F30bgpz1XXquycKrS1m81TDDFPA1avNGsb833Fiv3KRknPJFs8iL5_fXr_GGK5GPwSR9VrTJn84WRy7pzVejxn7C_fWiHww</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>FIRSOV, M.L.</creator><creator>GREEN, D.G.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980701</creationdate><title>Photoreceptor coupling in turtle retina</title><author>FIRSOV, M.L. ; GREEN, D.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-8d63f06a35f1a4165c278d173d0aa53c1944e8980a0bca9843d96952633270b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotin - analogs & derivatives</topic><topic>Cell coupling</topic><topic>Cone</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gap Junctions - physiology</topic><topic>Isoquinolines</topic><topic>Neurobiotin</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>Retina - anatomy & histology</topic><topic>Retina - physiology</topic><topic>Retinal Cone Photoreceptor Cells - cytology</topic><topic>Retinal Cone Photoreceptor Cells - physiology</topic><topic>Retinal Rod Photoreceptor Cells - cytology</topic><topic>Retinal Rod Photoreceptor Cells - physiology</topic><topic>Rod</topic><topic>Telodendria</topic><topic>Turtles</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Visual Pathways</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FIRSOV, M.L.</creatorcontrib><creatorcontrib>GREEN, D.G.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Visual neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FIRSOV, M.L.</au><au>GREEN, D.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photoreceptor coupling in turtle retina</atitle><jtitle>Visual neuroscience</jtitle><addtitle>Vis Neurosci</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>15</volume><issue>4</issue><spage>755</spage><epage>764</epage><pages>755-764</pages><issn>0952-5238</issn><eissn>1469-8714</eissn><abstract>Photoreceptors in the isolated turtle retina of two
species of turtle, Chelydra serpentina and
Pseudemus scripta elegans, were penetrated with double-barrel
electrodes. Physiological responses were recorded through
one barrel and Neurobiotin tracer was injected from the
other. Intracellular injection of Neurobiotin revealed
patterns of tracer-coupled photoreceptors. Both the patterns
of tracer coupling and the electrophysiology suggest a
high degree of specificity of connections. Rods seem to
be coupled only to rods and green and red cones seem to
be coupled to cones of the same spectral type. Receptive-field
profiles, measured with a thin, sharply focused slit of
light, often had well-defined peaks and troughs in sensitivity.
We have taken advantage of this observation and used the
position of a peak in sensitivity to locate the position
on the retina of a coupled cell. In one rod, it was possible
to correlate physiological and morphological data and to
show that the peaks in the physiological receptive field
occurred at positions on the retina where there were dye-coupled
cells. This provides direct evidence that gap junctions
produce the physiological coupling between rods.</abstract><cop>New York, NY</cop><pub>Cambridge University Press</pub><pmid>9682876</pmid><doi>10.1017/S0952523898154147</doi><tpages>10</tpages></addata></record> |
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| ispartof | Visual neuroscience, 1998-07, Vol.15 (4), p.755-764 |
| issn | 0952-5238 1469-8714 |
| language | eng |
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| source | Cambridge University Press |
| subjects | Animals Biological and medical sciences Biotin - analogs & derivatives Cell coupling Cone Eye and associated structures. Visual pathways and centers. Vision Fluorescent Dyes Fundamental and applied biological sciences. Psychology Gap Junctions - physiology Isoquinolines Neurobiotin Neurons - cytology Neurons - physiology Retina - anatomy & histology Retina - physiology Retinal Cone Photoreceptor Cells - cytology Retinal Cone Photoreceptor Cells - physiology Retinal Rod Photoreceptor Cells - cytology Retinal Rod Photoreceptor Cells - physiology Rod Telodendria Turtles Vertebrates: nervous system and sense organs Visual Pathways |
| title | Photoreceptor coupling in turtle retina |
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