Analysis of Antibody Cross-Reactivity in Experimental American Trypanosomiasis

Susceptible C3H/He mice were immunized with the avirulent Corpus Christi strain of Trypanosoma cruzi and subsequently infected with virulent Brazil stain organisms. Seventy days after infection sera were isolated and analyzed on western blots of electrophoretically separated T. cruzi antigens prepar...

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Bibliographic Details
Published in:The Journal of parasitology 1990-10, Vol.76 (5), p.698-702
Main Authors: Rosfjord, Edward C., Mikhail, Kelly Spears, Rowland, Edwin C., Powell, Malcolm R.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Protozoan - analysis
Antibodies, Protozoan - immunology
Antigens
Biological and medical sciences
Blotting, Western
Cellulose nitrate
Chagas disease
Chagas Disease - immunology
Cross reaction
Cross Reactions
Epitopes
Epitopes - immunology
Experimental protozoal diseases and models
Female
Immunology
Infections
Infectious diseases
Medical sciences
Mice
Mice, Inbred C3H
Molecular Weight
Parasitic diseases
Parasitology
Protozoal diseases
Trypanosoma cruzi - immunology
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Summary:Susceptible C3H/He mice were immunized with the avirulent Corpus Christi strain of Trypanosoma cruzi and subsequently infected with virulent Brazil stain organisms. Seventy days after infection sera were isolated and analyzed on western blots of electrophoretically separated T. cruzi antigens prepared from culture-form parasites (primarily epimastigotes). More than 25 bands were identified. The antibodies were fractionated by elution from various regions of western blots corresponding to average molecular weights of approximately >130, 77, 70, 60, 48, or 38 kDa. Each of these antibody preparations was then incubated with strips of nitrocellulose containing all of the electrophoretically separated T. cruzi, and cross-reactivity was determined. Antibodies isolated from the 130-, 77-, and 70-kDa regions all cross-reacted with each other. Antibodies eluted from the 60-kDa region bound antigens in the 60-, 70-, and the 77-kDa regions. More importantly, antibodies eluted from every region bound antigens in the 70-kDa region. Conversely, antibodies eluted from this region bound to antigens in all of the other regions. These data indicate the presence of (a) common antigenic epitope(s) in T. cruzi infections in these mice that is predominately found in the 70-kDa antigen-antibody complex on western blots.
ISSN:0022-3395
1937-2345
DOI:10.2307/3282985