Transgenic Nicotiana debneyii expressing viral coat protein are resistant to potato virus S infection

Research Station, Agriculture Canada, 6660 Northwest Marine Drive, Vancouver, British Columbia V6T 1X2, Canada The coat protein gene from potato virus S (PVS) was introduced into Nicotiana debneyii by leaf disc transformation using Agrobacterium tumefaciens . Transgenic plants expressing the viral c...

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Bibliographic Details
Published in:Journal of general virology 1990-09, Vol.71 (9), p.2167-2170
Main Authors: MacKenzie, Donald J, Tremaine, J. H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Capsid - genetics
Capsid - isolation & purification
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Genes, Viral
Immunoblotting
Microbiology
Plant Diseases
Plant Viruses - genetics
Plant Viruses - pathogenicity
Plants - genetics
potato virus S
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Solanum tuberosum
Virology
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Summary:Research Station, Agriculture Canada, 6660 Northwest Marine Drive, Vancouver, British Columbia V6T 1X2, Canada The coat protein gene from potato virus S (PVS) was introduced into Nicotiana debneyii by leaf disc transformation using Agrobacterium tumefaciens . Transgenic plants expressing the viral coat protein were highly resistant to subsequent infection by the ME strain of PVS as indicated by an absence of symptom development and a lack of accumulation of virus in both the inoculated and upper leaves. As in reported experiments with plants expressing potato virus X coat protein, plants expressing PVS coat protein were also protected from inoculation with PVS RNA. These results provide further evidence that coat protein-mediated protection for these two groups of viruses, which share similar genome organizations, may involve inhibition of some early event in infection other than or in addition to virus uncoating. Received 28 February 1990; accepted 22 May 1990.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-71-9-2167