Determination of nifedipine in human plasma by flow-injection tandem mass spectrometry

For use in clinical studies, a fast and sensitive assay method was developed for the determination of nifedipine in human plasma samples. The assay method is based on tandem mass spectrometry detection (HPLC–MS–MS). The effect of flow injection as well as HPLC separation on the results of the nifedi...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1998-06, Vol.710 (1), p.115-120
Main Authors: Dankers, Jan, van den Elshout, Jos, Ahr, Gertrude, Brendel, Erich, van der Heiden, Cees
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Calcium Channel Blockers - blood
Calibration
Chromatography, High Pressure Liquid - methods
Evaluation Studies as Topic
General pharmacology
Humans
Mass Spectrometry - methods
Medical sciences
Nifedipine
Nifedipine - blood
Pharmacology. Drug treatments
Reproducibility of Results
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Description
Summary:For use in clinical studies, a fast and sensitive assay method was developed for the determination of nifedipine in human plasma samples. The assay method is based on tandem mass spectrometry detection (HPLC–MS–MS). The effect of flow injection as well as HPLC separation on the results of the nifedipine determination were evaluated. The limit of quantification is 0.5 ng/ml and the accuracy (as determined by spiking recovery) was found to be good.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(98)00126-1