IL-10 Regulates Thrombus-Induced Vein Wall Inflammation and Thrombosis

Vein wall inflammation associated with venous thrombosis is mediated by an imbalance in proinflammatory as compared with antiinflammatory molecules. We hypothesize that IL-10 is an important antiinflammatory cytokine that influences vein wall inflammation and thrombus propagation during venous throm...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1998-08, Vol.161 (3), p.1471-1476
Main Authors: Downing, L. Joseph, Strieter, Robert M, Kadell, Amy M, Wilke, Carol A, Austin, John C, Hare, Bradley D, Burdick, Marie D, Greenfield, Lazar J, Wakefield, Thomas W
Format: Article
Language:English
Subjects:
Animals
Constriction, Pathologic
Dose-Response Relationship, Immunologic
Drug Administration Schedule
Immune Sera - administration & dosage
Inflammation - immunology
Inflammation - prevention & control
Injections, Intravenous
Interleukin-10 - administration & dosage
Interleukin-10 - genetics
Interleukin-10 - immunology
Interleukin-10 - physiology
Rats
Rats, Sprague-Dawley
Recombinant Proteins - administration & dosage
Thrombophlebitis - immunology
Thrombophlebitis - pathology
Thrombophlebitis - prevention & control
Thrombosis - immunology
Vena Cava, Inferior - pathology
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Summary:Vein wall inflammation associated with venous thrombosis is mediated by an imbalance in proinflammatory as compared with antiinflammatory molecules. We hypothesize that IL-10 is an important antiinflammatory cytokine that influences vein wall inflammation and thrombus propagation during venous thrombosis. To test this hypothesis a model of inferior vena caval thrombosis was used. Studies were performed at sacrifice 2 days after thrombus induction and included leukocyte morphometrics, myeloperoxidase activity, vein wall permeability, thrombus weight, and IL-10 ELISA analysis from the vein wall. IL-10 was elevated in the vein wall during venous thrombosis. Neutralization of IL-10 increased inflammation, while supplementation with rIL-10 demonstrated a dose- and time-dependent decrease in inflammation. Interestingly, a low 2.5-microg rIL-10 dose given at time of initiation of thrombosis most significantly decreased inflammation. Thrombus weight was importantly diminished by reconstitution of IL-10. These studies support an important role for IL-10 in the regulation of thrombus-associated inflammation and thrombosis and suggest that IL-10 could be used as a therapeutic agent in the treatment of venous thrombosis.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.161.3.1471