Plastid promoter utilization in a rice embryogenic cell culture

Plastid promoter utilization was characterized in rice by mapping transcript 5'-ends in samples derived from leaves and cultured embryogenic cells. We have found that rbcL, atpB and the rRNA operon are transcribed by the plastid-encoded plastid RNA polymerase (PEP), while clpP is transcribed by...

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Bibliographic Details
Published in:Current genetics 1998-07, Vol.34 (1), p.67-70
Main Authors: Silhavy, D, Maliga, P
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Base Sequence
Cells, Cultured
chloroplast DNA
chloroplasts
Chloroplasts - metabolism
chromosome mapping
cultured cells
DNA, Plant - chemistry
DNA-directed RNA polymerase
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
Endopeptidase Clp
enzyme activity
gene expression
Histone-Lysine N-Methyltransferase - genetics
leaves
messenger RNA
Nicotiana tabacum
nucleotide sequences
operon
Oryza - embryology
Oryza - genetics
Oryza sativa
Plant Leaves - chemistry
plastids
Plastids - genetics
promoter regions
Promoter Regions, Genetic
ribosomal RNA
RNA, Ribosomal, 16S - genetics
Sequence Alignment
Serine Endopeptidases
somatic embryogenesis
transcription (genetics)
Transcription, Genetic
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Summary:Plastid promoter utilization was characterized in rice by mapping transcript 5'-ends in samples derived from leaves and cultured embryogenic cells. We have found that rbcL, atpB and the rRNA operon are transcribed by the plastid-encoded plastid RNA polymerase (PEP), while clpP is transcribed by the nucleus-encoded plastid RNA polymerase (NEP) in both chloroplasts and the non-green plastids of embryogenic cultured cells. This finding is in contrast to reports on BY2 tobacco, in which NEP promoter activity in cultured cells was enhanced relative to leaves, facilitating identification of NEP promoters which are undetectable in chloroplasts. Therefore, it appears that activation of plastid NEP promoters in rice is not essential for adaptation to cell culture.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050367