Plastid promoter utilization in a rice embryogenic cell culture

• Published in: Current genetics 1998-07, Vol.34 (1), p.67-70
• Main Authors: Silhavy, D, Maliga, P
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphatases - genetics; Base Sequence; Cells, Cultured; chloroplast DNA; chloroplasts; Chloroplasts - metabolism; chromosome mapping; cultured cells; DNA, Plant - chemistry; DNA-directed RNA polymerase; DNA-Directed RNA Polymerases - genetics; DNA-Directed RNA Polymerases - metabolism; Endopeptidase Clp; enzyme activity; gene expression; Histone-Lysine N-Methyltransferase - genetics; leaves; messenger RNA; Nicotiana tabacum; nucleotide sequences; operon; Oryza - embryology; Oryza - genetics; Oryza sativa; Plant Leaves - chemistry; plastids; Plastids - genetics; promoter regions; Promoter Regions, Genetic; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Alignment; Serine Endopeptidases; somatic embryogenesis; transcription (genetics); Transcription, Genetic
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/s002940050367

Plastid promoter utilization was characterized in rice by mapping transcript 5'-ends in samples derived from leaves and cultured embryogenic cells. We have found that rbcL, atpB and the rRNA operon are transcribed by the plastid-encoded plastid RNA polymerase (PEP), while clpP is transcribed by the nucleus-encoded plastid RNA polymerase (NEP) in both chloroplasts and the non-green plastids of embryogenic cultured cells. This finding is in contrast to reports on BY2 tobacco, in which NEP promoter activity in cultured cells was enhanced relative to leaves, facilitating identification of NEP promoters which are undetectable in chloroplasts. Therefore, it appears that activation of plastid NEP promoters in rice is not essential for adaptation to cell culture.