Ecto-ATP diphosphohydrolase/CD39 is overexpressed in differentiated human melanomas

Ecto-ATPase activities of melanocytes and human melanoma cell lines differing in the stage of progression were compared. A dramatic increase in ecto-ATPase activity above the level of normal melanocytes was demonstrated in the differentiated melanomas and was followed by a gradual decrease with tumo...

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Bibliographic Details
Published in:FEBS letters 1998-07, Vol.430 (3), p.227-230
Main Authors: Dzhandzhugazyan, Karine N, Kirkin, Alexei F, thor Straten, Per, Zeuthen, Jesper
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases
Adhesion
Antigens, CD - genetics
Antigens, CD - metabolism
Apyrase - antagonists & inhibitors
Apyrase - genetics
Apyrase - metabolism
ATPDase, ATP diphosphohydrolase
Biomarkers, Tumor - antagonists & inhibitors
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
CD39
Cell Differentiation
Cell Line
CTL, cytotoxic T-lymphocytes
Differentiation marker
Disease Progression
Ecto-ATPase
Enzyme Inhibitors - pharmacology
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Human melanoma
Humans
Immune escape mechanism
mABs, monoclonal antibodies
Melanocytes
Melanoma - enzymology
Melanoma - genetics
Melanoma - pathology
RNA, Messenger - analysis
RNA, Neoplasm - analysis
RT-PCR, reverse transcriptase-coupled polymerase chain reaction
Tumor Cells, Cultured
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Summary:Ecto-ATPase activities of melanocytes and human melanoma cell lines differing in the stage of progression were compared. A dramatic increase in ecto-ATPase activity above the level of normal melanocytes was demonstrated in the differentiated melanomas and was followed by a gradual decrease with tumor progression. The characteristics of this enzymatic activity were consistent with CD39/ecto-ATP diphosphohydrolase (ATPDase) which was found to be the major ecto-ATP-hydrolysing enzyme in melanomas. Indeed, the expression of CD39 and the level of CD39 mRNA followed a similar pattern. Since CD39 is known to regulate homotypic adhesion and, supposedly, affects the disaggregation step, we suggest that overexpression of CD39 may enable tumor cells to reduce contacts with T-lymphocytes and escape from immunological recognition.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)00603-6