Human osteoblast-like cells respond not only to the extracellular calcium concentration but also to its changing rate

The effect of extracellular calcium on human osteoblast-like cells (HOS) has been demonstrated. An experimental setup was used for applying defined rates of change in the extracellular calcium concentration. The intracellular calcium concentration was monitored using the fluorescence dye fura-2. HOS...

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Bibliographic Details
Published in:European biophysics journal 1998-01, Vol.27 (4), p.411-416
Main Authors: Habel, B, Glaser, R
Format: Article
Language:English
Subjects:
Biophysical Phenomena
Biophysics
Bone Remodeling - physiology
Calcium
Calcium (extracellular)
Calcium (intracellular)
Calcium - metabolism
Calcium - pharmacology
Cells
Enzyme Inhibitors - pharmacology
Extracellular Space - metabolism
Fluorescence
Fura-2
GTP-Binding Proteins - metabolism
Guanine nucleotide-binding protein
Humans
Inositol 1,4,5-Trisphosphate - metabolism
Intracellular Fluid - metabolism
Kinetics
Neomycin
Neomycin - pharmacology
Osteoblasts - drug effects
Osteoblasts - metabolism
Osteosarcoma - metabolism
Proteins
Signal transduction
Space life sciences
Tumor Cells, Cultured
Type C Phospholipases - antagonists & inhibitors
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Summary:The effect of extracellular calcium on human osteoblast-like cells (HOS) has been demonstrated. An experimental setup was used for applying defined rates of change in the extracellular calcium concentration. The intracellular calcium concentration was monitored using the fluorescence dye fura-2. HOS cells showed qualitatively different responses of the intracellular calcium concentration to changes of the extracellular calcium concentration depending on its changing rate. A small rate caused only a small and slow increase of the intracellular calcium concentration, whereas faster changes are able to cause a rapid transient increase followed by a sustained elevation of the internal calcium level. Surprisingly, both an increasing as well as a decreasing external calcium concentration is able to cause cellular responses. These signals could be reduced by the IP3-inhibitor neomycin. We propose that the G-protein dependent signalling pathway of HOS cells can not only sense the extracellular calcium concentration but also its time derivative.
ISSN:0175-7571
1432-1017
DOI:10.1007/s002490050149