Involvement of a Gardos-type potassium channel in head activator-induced mitosis of BON cells

The human neuroendocrine cell line BON was used to study second messengers involved in signal transduction for entry into mitosis. BON cells produce the neuropeptide head activator (HA) and use it as autocrine growth factor. HA stimulates BON cell proliferation by triggering entry into mitosis. HA-i...

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Bibliographic Details
Published in:European journal of cell biology 1998-06, Vol.76 (2), p.119-124
Main Authors: Kayser, Stefan T., Ulrich, Henning, Chica Schaller, H.
Format: Article
Language:English
Subjects:
Autocrine Communication
Blotting, Northern
BON cells
Calcium - metabolism
Cell Count - drug effects
Cell Cycle
Clotrimazole - pharmacology
Cyclic AMP - metabolism
Flow Cytometry
Gardos channel
Growth Substances - physiology
GTP-Binding Protein alpha Subunits, Gi-Go - metabolism
head activator
Humans
Intermediate-Conductance Calcium-Activated Potassium Channels
Mitosis - drug effects
mitotic
Neuropeptides - agonists
Neuropeptides - metabolism
Neuropeptides - pharmacology
Pancreas - cytology
pancreatic carcinoid
Potassium - metabolism
Potassium Channel Blockers
Potassium Channels - genetics
Potassium Channels - physiology
Potassium Channels, Calcium-Activated
Pyrrolidonecarboxylic Acid - analogs & derivatives
Radioimmunoassay
Second Messenger Systems - drug effects
Signal Transduction
Tumor Cells, Cultured
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Summary:The human neuroendocrine cell line BON was used to study second messengers involved in signal transduction for entry into mitosis. BON cells produce the neuropeptide head activator (HA) and use it as autocrine growth factor. HA stimulates BON cell proliferation by triggering entry into mitosis. HA-induced mitosis is mediated by an inhibitory G protein, the action of which is blocked by pertussis toxin. HA signaling requires inhibition of the cAMP pathway, calcium inftux, and hyperpolarization of cells. The latter is a very important and sensitive step involving a calcium-activated potassium channel. Cell cycle progression and proliferation of BON cells are most efficiently inhibited with specific inhibitors of this potassium channel. Pharmacology and RNA analysis suggest identity with the recently cloned Gardos-type potassium channel.
ISSN:0171-9335
1618-1298
DOI:10.1016/S0171-9335(98)80024-1