Occurrence of introns in the 16S rRNA genes of members of the genus Thermoproteus

Multiple introns were detected in the 16S rRNA gene of newly isolated Thermoproteus species strains IC-033 and IC-061 and Thermoproteus neutrophilus JCM 9278. In the 16S rRNA gene of strain IC-033, five introns of 627, 762, 636, 33, and 682 bp existed after positions 548, 781, 1092, 1205, and 1213 (...

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Bibliographic Details
Published in:Archives of microbiology 1998-09, Vol.170 (3), p.155-161
Main Authors: ITOH, T, SUZUKI, K.-I, NAKASE, T
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Evolution, Molecular
Frameshift Mutation
Fundamental and applied biological sciences. Psychology
Genes, Archaeal
Genetics
Introns
Japan
Microbiology
Molecular Sequence Data
Open Reading Frames
Phylogeny
Polymerase Chain Reaction
RNA, Archaeal - genetics
RNA, Ribosomal, 16S - genetics
rRNA Operon - genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Space life sciences
Thermoproteaceae - classification
Thermoproteaceae - genetics
Thermoproteus
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Summary:Multiple introns were detected in the 16S rRNA gene of newly isolated Thermoproteus species strains IC-033 and IC-061 and Thermoproteus neutrophilus JCM 9278. In the 16S rRNA gene of strain IC-033, five introns of 627, 762, 636, 33, and 682 bp existed after positions 548, 781, 1092, 1205, and 1213 (according to the Escherichia coli numbering system), respectively. Likewise, strain IC-061 possessed 764-, 32-, and 688-bp introns after positions 781, 1205, and 1213, respectively; and T. neutrophilus JCM 9278 had 34- and 663-bp introns after positions 1205 and 1213, respectively. All the introns carried the putative intron core structures consisting of a bulge-helix-bulge motif and a long stable stem. The large introns carried open reading frames containing the LAGLI-DADG-like motifs in their terminal inserts; however, three out of four large introns of strain IC-033 seemed to incur frameshift mutations. Occurrence of introns at the same insertion sites in the three strains would allow tracing of the evolutionary movements of these introns.
ISSN:0302-8933
1432-072X
DOI:10.1007/s002030050628