Prolyl Oligopeptidase: An Unusual β-Propeller Domain Regulates Proteolysis

Prolyl oligopeptidase is a large cytosolic enzyme that belongs to a new class of serine peptidases. The enzyme is involved in the maturation and degradation of peptide hormones and neuropeptides, which relate to the induction of amnesia. The 1.4 Å resolution crystal structure is presented here. The...

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Bibliographic Details
Published in:Cell 1998-07, Vol.94 (2), p.161-170
Main Authors: Fueloep, V, Boecskei, Z, Polgar, L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Binding Sites
Crystallography, X-Ray
Models, Molecular
Molecular Sequence Data
Protein Folding
Protein Structure, Secondary
Protein Structure, Tertiary
Serine Endopeptidases - chemistry
Swine
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Summary:Prolyl oligopeptidase is a large cytosolic enzyme that belongs to a new class of serine peptidases. The enzyme is involved in the maturation and degradation of peptide hormones and neuropeptides, which relate to the induction of amnesia. The 1.4 Å resolution crystal structure is presented here. The enzyme contains a peptidase domain with an α/β hydrolase fold, and its catalytic triad (Ser554, His680, Asp641) is covered by the central tunnel of an unusual β propeller. This domain makes prolyl oligopeptidase an oligopeptidase by excluding large structured peptides from the active site. In this way, the propeller protects larger peptides and proteins from proteolysis in the cytosol. The structure is also obtained with a transition state inhibitor, which may facilitate drug design to treat memory disorders.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)81416-6