Gene complementing xeroderma pigmentosum group A cells maps to distal human chromosome 9q

Phenotypic complementation of xeroderma pigmentosum group A (XP-A) cells by microcell-mediated transfer of a single rearranged neo-tagged human chromosome from a human-mouse somatic cell hybrid designated K3SUB1A9-3 was reported previously. Extended growth of this human-mouse hybrid in culture led t...

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Bibliographic Details
Published in:Somatic cell and molecular genetics 1990-07, Vol.16 (4), p.395-400
Main Authors: HENNING, K. A, SCHULTZ, R. A, SEKHON, G. S, FRIEDBERG, E. C
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Southern
Cell Line
Chromosome Mapping
Chromosomes, Human, Pair 9
Classical genetics, quantitative genetics, hybrids
Cytogenetics
Fundamental and applied biological sciences. Psychology
Genetic Complementation Test
Genetics of eukaryotes. Biological and molecular evolution
Humans
Hybrid Cells
Mice
Transfection
Translocation, Genetic
Vertebrata
Xeroderma Pigmentosum - genetics
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Summary:Phenotypic complementation of xeroderma pigmentosum group A (XP-A) cells by microcell-mediated transfer of a single rearranged neo-tagged human chromosome from a human-mouse somatic cell hybrid designated K3SUB1A9-3 was reported previously. Extended growth of this human-mouse hybrid in culture led to deletion of the small arm of the human chromosome, with concomitant loss of complementing ability when introduced into XP-A cells by microcell-mediated chromosome transfer. Cytogenetic analysis of both hybrids suggests that the complementing locus is on chromosome 9q22.2-q34.3, and Southern blot analysis confirms the presence of distal chromosome 9q sequences.
ISSN:0740-7750
1572-9931
DOI:10.1007/BF01232467