Separation of lac dye components by high-speed counter-current chromatography

High-speed counter-current chromatography has been successfully applied to the separation of the lac dye components. A 25-mg quantity of the sample was separated using a two-phase solvent system composed of tert.-butyl methyl ether– n-butanol–acetonitrile–water (2:2:1:5). The fractions were analyzed...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-07, Vol.813 (1), p.71-77
Main Authors: Oka, Hisao, Ito, Yuko, Yamada, Sadaji, Kagami, Tadaaki, Hayakawa, Junko, Harada, Ken-ichi, Atsumi, Eiichiro, Suzuki, Masanao, Suzuki, Makoto, Odani, Hiroko, Akahori, Setsuko, Maeda, Kenji, Nakazawa, Hiroyuki, Ito, Yoichiro
Format: Article
Language:English
Subjects:
Azo Compounds - isolation & purification
Biological and medical sciences
Chromatography
Countercurrent Distribution
Dyes
Food additives
Food Coloring Agents - isolation & purification
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Indicators and Reagents
Lac dye
Laccaic acids
Mass Spectrometry
Solvents
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Summary:High-speed counter-current chromatography has been successfully applied to the separation of the lac dye components. A 25-mg quantity of the sample was separated using a two-phase solvent system composed of tert.-butyl methyl ether– n-butanol–acetonitrile–water (2:2:1:5). The fractions were analyzed by high-performance liquid chromatography and electrospray tandem mass spectrometry. The separation yielded 2.6 mg of 97.2% pure laccaic acid C, 9.5 mg of 98.1% pure laccaic acid A, 3.6 mg of 98.2% pure laccaic acid B, and 0.5 mg of a 95.0% pure anthraquinonedicarboxylic acid with a molecular mass of 360.
ISSN:0021-9673
DOI:10.1016/S0021-9673(98)00311-2