A newly established human acute lymphoblastic leukemia cell line with characteristics of the earliest B-cell maturation

A new human acute lymphoblastic leukemia (ALL) cell line, designated HBL-3, was established from the bone marrow of a patient with non-T-ALL. The HBL-3 cell line expressed B4 (CD 19), BA-1 (CD 24) and HLA-DR antigens, but not surface immunoglobulin (SIg) or cytoplasmic immunoglobulin (CIg). The cell...

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Bibliographic Details
Published in:Virchows Archiv. Abteilung B. Zellpathologie 1990-01, Vol.59 (2), p.107-113
Main Authors: ABE, M, NAKAMURA, N, FUKUHARA, S, HAYASHI, T, KAWAKAMI, K, KITA, K, KINOSHITA, T, OHSATO, T, WAKASA, H
Format: Article
Language:English
Subjects:
B-Lymphocytes - pathology
Biological and medical sciences
Bone Marrow - pathology
Cell Nucleus - ultrastructure
Child
Chromosome Aberrations
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 9
Cytoplasm - ultrastructure
DNA Nucleotidylexotransferase - analysis
Female
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Hematologic and hematopoietic diseases
Humans
Immunophenotyping
Karyotyping
Medical sciences
Microscopy, Electron
Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology
Translocation, Genetic
Tumor Cells, Cultured
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Summary:A new human acute lymphoblastic leukemia (ALL) cell line, designated HBL-3, was established from the bone marrow of a patient with non-T-ALL. The HBL-3 cell line expressed B4 (CD 19), BA-1 (CD 24) and HLA-DR antigens, but not surface immunoglobulin (SIg) or cytoplasmic immunoglobulin (CIg). The cell line lacked the common acute lymphoblastic leukemia antigen (CALLA) and antigenic markers characteristic of T-cell and myeloid cell lineages. The HBL-3 cells had structural rearrangements of both the homologous chromosome 9s, including a translocation with chromosome 1 which has been reported in a patient with common ALL. The cell line had rearranged immunoglobulin heavy chain genes but retained germ-line kappa light chain genes and germ-line T-cell receptor beta- and gamma-chain genes. The HBL-3 cell line was strongly positive for terminal deoxynucleotidyl transferase (TdT). These findings indicate that the HBL-3 cell line is derived from the earliest B-cell committed to B-cell lineage.
ISSN:0340-6075
0042-6431
DOI:10.1007/BF02899394