Isoform-Specific cDNAs for Human Embryonic, Neonatal, and Slow Skeletal Myosin Heavy Chains

A series of cDNA fragments encoding immunodetectable portions of the human slow/beta, neonatal, and embryonic isoforms of myosin heavy chain (MHC) were isolated from a human fetal muscle cDNA expression library. A 6 kb fragment isolated on a secondary screen represents the first cloned cDNA encoding...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1990, Vol.599 (1), p.119-126
Main Authors: STEDMAN, HANSELL H., KELLY, ALAN M., RUBINSTEIN, NEAL A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
DNA - genetics
DNA - isolation & purification
Embryo, Mammalian
Gene Library
Humans
Infant, Newborn
Molecular Sequence Data
Muscles - embryology
Muscles - physiology
Myosin Subfragments - genetics
Myosins - genetics
Rats
Restriction Mapping
Sequence Homology, Nucleic Acid
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Summary:A series of cDNA fragments encoding immunodetectable portions of the human slow/beta, neonatal, and embryonic isoforms of myosin heavy chain (MHC) were isolated from a human fetal muscle cDNA expression library. A 6 kb fragment isolated on a secondary screen represents the first cloned cDNA encoding a full-length vertebrate MHC (the human embryonic isoform). In the 3'-untranslated regions, 70-80% nucleotide sequence homology exists among orthologous human and rat cDNAs, whereas the homology is less than 65% among the paralogous cDNAs. Furthermore, approximately the same level of untranslated sequence conservation is observed at the 5'-terminus of the embryonic transcript. These results suggest that for both the 3'- and the 5'-untranslated domains, the rate of evolutionary sequence divergence is limited by functional constraints.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1990.tb42370.x