Neutrophil-Free Radical Generation and Enzymatic Antioxidants in Migraine Patients

The present study was undertaken to elucidate the role of circulating neutrophils if any in oxidative stress in migraine by evaluating free radical generation and activities of enzymatic antioxidants in the blood in 55 patients with migraine and 60 healthy controls. Free radical generation was asses...

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Bibliographic Details
Published in:Cephalalgia 2004-01, Vol.24 (1), p.37-43
Main Authors: Shukla, R, Barthwal, MK, Srivastava, N, Sharma, P, Raghavan, SAV, Nag, D, Srimal, RC, Seth, PK, Dikshit, M
Format: Article
Language:English
Subjects:
Adult
Age Factors
Antioxidants - analysis
Blood Platelets - enzymology
Catalase
Catalase - blood
Female
Free Radicals - blood
glutathione peroxidase
Glutathione Peroxidase - blood
Humans
Male
migraine
Migraine Disorders - blood
Neutrophil Activation
neutrophils
Neutrophils - enzymology
Oxidative Stress
Oxidoreductases - blood
Reference Values
Severity of Illness Index
Sex Factors
Statistics as Topic
superoxide dismutase
Superoxide Dismutase - blood
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Summary:The present study was undertaken to elucidate the role of circulating neutrophils if any in oxidative stress in migraine by evaluating free radical generation and activities of enzymatic antioxidants in the blood in 55 patients with migraine and 60 healthy controls. Free radical generation was assessed by flow cytometry, while activity of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) was estimated in blood polymorphonuclear neutrophils (PMNs) by standard procedures. Platelet SOD was also measured. No significant change was found in free radical generation and in the activity of catalase, SOD and GPx in migraine patients. Univariate analysis of PMN catalase level revealed that migraineurs with a positive family history had significantly lower catalase activity compared with those with a negative family history. No correlation was found in the activity of antioxidant enzymes with age, duration of disease, time since last attack and headache index. The platelet SOD also did not show any significant change in patients of migraine without aura. Platelet aggregation in the presence or absence of PMNs was also not altered significantly. Thus the findings of the present study suggest that neutrophils are not the cause of oxidative stress observed in migraine patients.
ISSN:0333-1024
1468-2982
DOI:10.1111/j.1468-2982.2004.00631.x