Detection and characterization of measles virus strains in cases of subacute sclerosing panencephalitis in Croatia

Two cases of subacute sclerosing panencephalitis (SSPE), diagnosed in Croatia in 2002, were investigated. The coding regions of the matrix (M), hemagglutinin (H) and nucleoprotein (N) genes of measles virus were sequenced following direct RT-PCR amplification of viral RNA extracted from brain tissue...

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Bibliographic Details
Published in:Virus research 2004, Vol.99 (1), p.51-56
Main Authors: Forčić, Dubravko, Baričević, Marijana, Zgorelec, Renata, Kružić, Vjekoslava, Kaić, Bernard, Marina, Branka Marušić Della, Šojat, Ljerka Cvitanović, Tešović, Goran, Mažuran, Renata
Format: Article
Language:English
Subjects:
Brain - virology
Child
Child, Preschool
Codon, Nonsense
Croatia
Female
Genes, Viral
Genotype
Hemagglutinins, Viral - genetics
Humans
Male
Measles genotype
Measles virus
Measles virus - classification
Measles virus - genetics
Measles virus - isolation & purification
Mutation, Missense
Mutations
Nucleoproteins - genetics
Phylogeny
Point Mutation
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - chemistry
RNA, Viral - isolation & purification
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
SSPE
Subacute Sclerosing Panencephalitis - virology
Viral Matrix Proteins - genetics
Viral Proteins - genetics
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Summary:Two cases of subacute sclerosing panencephalitis (SSPE), diagnosed in Croatia in 2002, were investigated. The coding regions of the matrix (M), hemagglutinin (H) and nucleoprotein (N) genes of measles virus were sequenced following direct RT-PCR amplification of viral RNA extracted from brain tissue. Phylogenetic analysis of the sequences of H and N genes, showed that both strains belonged to genotype D6. No vaccine strain was detected although both patients had been previously immunized. The comparison of analyzed sequences of two SSPE causative viruses with corresponding sequences of D6 genotype and with each other revealed a number of mutations in N and H gene sequences. In comparison to the Edmonston reference strain, the M gene of the SSPE viruses showed the characteristic biased hypermutation and a premature termination codon in one of the patients.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2003.10.003