High density microcarrier culture with a new device which allows oxygenation and perfusion of microcarrier cultures

A novel system useful for aeration and cell retention in continuous perfused microcarrier cultures is described. The system is based on a vibrating cage that separates cells and microcarriers from the oxygenation chamber and allows gas bubble free oxygen transfer. In the cultivation of monkey kidney...

Full description

Saved in:
Bibliographic Details
Published in:Cytotechnology (Dordrecht) 1990-01, Vol.3 (1), p.39-42
Main Authors: Reiter, M, Weigang, F, Ernst, W, Katinger, H W
Format: Article
Language:English
Subjects:
Animals
Biotechnology
Biotechnology - instrumentation
Cell Division
Cell Movement
Cells, Cultured
Equipment Design
Oxygen Consumption
Vero Cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A novel system useful for aeration and cell retention in continuous perfused microcarrier cultures is described. The system is based on a vibrating cage that separates cells and microcarriers from the oxygenation chamber and allows gas bubble free oxygen transfer. In the cultivation of monkey kidney cells (VERO) on gelatin coated microcarriers, using different concentrations (5, 10 and 15 g Cytodex 3/liter) cell densities up to 10(7) cells per ml were obtained. The described system is scaleable.
ISSN:0920-9069
1573-0778
DOI:10.1007/BF00365264