A continuous multistage roller reactor for animal cell culture: 1. Patterns of growth, production and catabolism of a murine hybridoma

A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each seg...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 1990-05, Vol.3 (3), p.253-258
Main Authors: Borth, N, Steindl, F, Weigang, F, Reiter, M, Katinger, H
Format: Article
Language:English
Subjects:
Alanine - metabolism
Animals
Antibodies, Monoclonal - biosynthesis
Biotechnology
Cell Division
Glucose - metabolism
Glutamine - metabolism
Hybridomas - cytology
Hybridomas - metabolism
Kinetics
Methods
Mice
Pyruvates - metabolism
Pyruvic Acid
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Summary:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high cell densities of up to 10(7) cells/ml were achieved due to cell sedimentation. The model character of the reactor was taken to determine critical values of substrate concentrations that influence production rates and result in an adaptation of metabolism.
ISSN:0920-9069
1573-0778
DOI:10.1007/BF00365489