Calbindin D-28k distribution in odontoblasts underneath tertiary dentine in human carious teeth

Objective: The aim of this study was to examine the calbindin D-28k immunoreactivity in carious teeth to know whether this protein may have a function in tertiary dentine formation. Methods: Human extracted teeth with or without carious lesions were immersion-fixed with Zamboni fixative, demineraliz...

Full description

Saved in:
Bibliographic Details
Published in:Archives of oral biology 2004, Vol.49 (1), p.37-43
Main Authors: Itota, Toshiyuki, Tashiro, Yoko, Torii, Yasuhiro, Nishitani, Yoshihiro, McCabe, John F, Yoshiyama, Masahiro
Format: Article
Language:English
Subjects:
Adult
Calbindin D-28k
Calbindins
Caries
Densitometry
Dental Caries - metabolism
Dental Caries - pathology
Dentin - metabolism
Dentin - pathology
Dentistry
Humans
Immunohistochemistry
Immunohistochemistry - methods
Odontoblasts
Odontoblasts - chemistry
S100 Calcium Binding Protein G - analysis
Staining and Labeling - methods
Tertiary dentine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective: The aim of this study was to examine the calbindin D-28k immunoreactivity in carious teeth to know whether this protein may have a function in tertiary dentine formation. Methods: Human extracted teeth with or without carious lesions were immersion-fixed with Zamboni fixative, demineralized in 4.13% EDTA solution (pH 7.4), frozen-sectioned, and processed for calbindin immunoreactivity and hematoxylin-eosin stain. The intensity of the immunostaining was evaluated by quantitative densitometry. Results: In intact teeth, numerous odontoblasts were aligned underneath the secondary dentine and their cell bodies showed the immunoreactivity. In carious teeth, tertiary dentine had poor- or rich tubular patterns under the carious lesion. Underneath the tubule-poor tertiary dentine, distinct odontoblasts could not be seen at the central site. However, some cells with a flat appearance were located at this site and were immunonegative for calbindin D-28k. On the other hand, columnar odontoblasts were seen at the peripheral site, and their cell bodies and processes showed strong immunoreactivity. Underneath the tubule-rich tertiary dentine, columnar odontoblasts were abundantly distributed, and the strong immunoreactivity was observed in their cell bodies and processes. The immunoreactivity in odontoblasts underneath the tertiary dentine with poor or rich tubular pattern was more intense than that for the secondary dentine in intact teeth ( P
ISSN:0003-9969
1879-1506
DOI:10.1016/S0003-9969(03)00183-3