Template-Directed Assembly of Metallosalen-DNA Hairpin Conjugates

Encoded assembly: Hairpin metallosalen–DNA was assembled from two salicylaldehyde‐modified, complementary oligonucleotides by DNA template‐directed synthesis. Assembly reactions in the absence of divalent metal ions produced a new class of metal‐free salen–DNA hairpin constructs. Metallosalen–DNA ha...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2004-01, Vol.5 (1), p.127-129
Main Authors: Czlapinski, Jennifer L., Sheppard, Terry L.
Format: Article
Language:English
Subjects:
Circular Dichroism
Cross-Linking Reagents
DNA - chemistry
DNA hairpin
Electrophoresis, Polyacrylamide Gel
Ethylenediamines - chemistry
Hot Temperature
Hydrogen-Ion Concentration
metal complex
Metallosalen
metallosalen-DNA
Nickel - chemistry
Nucleic Acid Conformation
Nucleic Acid Denaturation
nucleic acids
Protein Denaturation
Spectrophotometry, Ultraviolet
template synthesis
Templates, Genetic
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Summary:Encoded assembly: Hairpin metallosalen–DNA was assembled from two salicylaldehyde‐modified, complementary oligonucleotides by DNA template‐directed synthesis. Assembly reactions in the absence of divalent metal ions produced a new class of metal‐free salen–DNA hairpin constructs. Metallosalen–DNA hairpin conjugates demonstrated high B‐form duplex stability, which may facilitate structural studies of metallosalen–DNA conjugates.
ISSN:1439-4227
1439-7633
1439-4227
DOI:10.1002/cbic.200300776