Subnanomolar Cyanide Detection at Polyphenol Oxidase/Clay Biosensors

A novel, inexpensive, and simple amperometric biosensor based on immobilization of polyphenol oxidase (PPO) into Zn−Al layered double hydroxides, also called anionic clays, is applied for determination of cyanide. The detection of cyanide was performed via its inhibiting action on the PPO electrode....

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2004-01, Vol.76 (1), p.178-183
Main Authors: Shan, Dan, Mousty, Christine, Cosnier, Serge
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Catechol Oxidase - analysis
Chemistry
Cyanides - analysis
Electrochemical methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Nanotechnology - methods
Various methods and equipments
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Summary:A novel, inexpensive, and simple amperometric biosensor based on immobilization of polyphenol oxidase (PPO) into Zn−Al layered double hydroxides, also called anionic clays, is applied for determination of cyanide. The detection of cyanide was performed via its inhibiting action on the PPO electrode. Measurement was carried out with 3,4-dihydroxyphenylacetic acid as enzyme substrate, the enzymatically generated quinoid products being electroreduced at −0.2 V. An extremely sensitive detection limit (0.1 nM) was obtained for cyanide. Enzyme immobilization into an anionic exchanger clay seems to cause an increase in cyanide inhibition effects because of anion accumulation in the clay matrix.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac034713m