FKBP12-Rapamycin-associated Protein or Mammalian Target of Rapamycin (FRAP/mTOR) Localization in the Endoplasmic Reticulum and the Golgi Apparatus

FKBP12-rapamycin-associated protein (FRAP) or mammalian target of rapamycin (mTOR) and its effector proteins form a critical signaling pathway that regulates eukaryotic cell growth and proliferation. Although the protein components in this pathway have begun to be identified, little is known about t...

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Bibliographic Details
Published in:The Journal of biological chemistry 2004-01, Vol.279 (1), p.772-778
Main Authors: Drenan, Ryan M., Liu, Xiangyu, Bertram, Paula G., Zheng, X.F.Steven
Format: Article
Language:English
Subjects:
Animals
Carrier Proteins
Endoplasmic Reticulum - physiology
Golgi Apparatus - physiology
HeLa Cells
Humans
Mammals
Phosphotransferases (Alcohol Group Acceptor) - analysis
Phosphotransferases (Alcohol Group Acceptor) - physiology
Protein Kinases - analysis
Protein Kinases - physiology
Recombinant Proteins - analysis
Recombinant Proteins - metabolism
Tacrolimus Binding Protein 1A - analysis
Tacrolimus Binding Protein 1A - physiology
TOR Serine-Threonine Kinases
Transfection
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Summary:FKBP12-rapamycin-associated protein (FRAP) or mammalian target of rapamycin (mTOR) and its effector proteins form a critical signaling pathway that regulates eukaryotic cell growth and proliferation. Although the protein components in this pathway have begun to be identified, little is known about their subcellular localization or the physiological significance of their localization. By immunofluorescence, we find that both endogenous and recombinant FRAP/mTOR proteins show localization predominantly in the endoplasmic reticulum (ER) and the Golgi apparatus. Consistent with this finding, FRAP/mTOR is cofractionated with calnexin, an ER marker protein. Biochemical characterization suggests that FRAP/mTOR is a peripheral ER/Golgi protein with tight membrane association. Finally, we have identified domains of FRAP/mTOR which may mediate its association with the ER and the Golgi apparatus.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M305912200