Effect of semen collection practices on sperm characteristics before and after storage and on fertility of stallions

This study analyzed effects of different methods and intervals of semen collection on the quantity and quality of fresh, cool-stored, and frozen–thawed sperm and fertility of AI stallions. In Experiment 1, ejaculates were obtained from six stallions (72 ejaculates per stallion) using fractionated ve...

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Bibliographic Details
Published in:Theriogenology 2004-02, Vol.61 (4), p.769-784
Main Authors: Sieme, H, Katila, T, Klug, E
Format: Article
Language:English
Subjects:
Animals
Cell Membrane - ultrastructure
Cryopreservation
Ejaculation
Ejaculation frequency
Female
Fertility
Horses - physiology
Hot Temperature
libido
Male
male fertility
natural mating
Pregnancy
pregnancy rate
Semen collection
Semen Preservation - methods
Semen Preservation - veterinary
Sperm Count
Sperm Motility
Sperm quality
spermatozoa
Spermatozoa - physiology
Spermatozoa - ultrastructure
Stallion
storage quality
Time Factors
Tissue and Organ Harvesting - methods
Tissue and Organ Harvesting - veterinary
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Summary:This study analyzed effects of different methods and intervals of semen collection on the quantity and quality of fresh, cool-stored, and frozen–thawed sperm and fertility of AI stallions. In Experiment 1, ejaculates were obtained from six stallions (72 ejaculates per stallion) using fractionated versus non-fractionated semen collection techniques. Initial sperm quality of the first three jets of the ejaculate was not different from that of total ejaculates. Centrifugation of sperm-rich fractions before freezing improved post-thaw motility and sperm membrane integrity when compared to non-centrifuged sperm-rich fractions or non-fractionated centrifuged ejaculates ( P
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(03)00251-6