Over-expression of parvalbumin in transgenic mice rescues motoneurons from injury-induced cell death

• Published in: Neuroscience 2004, Vol.123 (2), p.459-466
• Main Authors: Dekkers, J, Bayley, P, Dick, J.R.T, Schwaller, B, Berchtold, M.W, Greensmith, L
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; axotomy; Biological and medical sciences; calcium-binding proteins; Cell Death; Female; Fundamental and applied biological sciences. Psychology; glutamate excitotoxicity; Immunohistochemistry; Male; Mice; Mice, Transgenic; Motor Neurons - metabolism; Motor Neurons - pathology; Muscle, Skeletal - innervation; Muscle, Skeletal - physiopathology; Nerve Crush; Nerve Degeneration; Nerve Regeneration; Parvalbumins - biosynthesis; Parvalbumins - genetics; Polymerase Chain Reaction; Recovery of Function; Sciatic Nerve - physiology; Spinal Cord - metabolism; Spinal Cord - pathology; Vertebrates: nervous system and sense organs
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2003.07.013

Following nerve injury in neonatal rats, a large proportion of motoneurons die, possibly as a consequence of an increase in vulnerability to the excitotoxic effects of glutamate. Calcium-dependent glutamate excitotoxicity is thought to play a significant role not only in injury-induced motoneuron death, but also in motoneuron degeneration in diseases such as amyotrophic lateral sclerosis (ALS). Motoneurons are particularly vulnerable to calcium influx following glutamate receptor activation, as they lack a number of calcium binding proteins, such as calbindin-D 28k and parvalbumin. Therefore, it is possible that increasing the ability of motoneurons to buffer intracellular calcium may protect them from cell death and prevent the decline in motor function that usually occurs as a consequence of motoneuron loss. In this study we have tested this possibility by examining the effect of neonatal axotomy on motoneuron survival and muscle force production in normal and transgenic mice that over-express parvalbumin in their motoneurons. The sciatic nerve was crushed in one hindlimb of new-born transgenic and wildtype mice. The effect on motoneuron survival was assessed 8 weeks later by retrograde labelling of motoneurons innervating the tibialis anterior muscle. Following nerve injury in wildtype mice, only 20.2% (±2.2, S.E.M.; n=4) of injured motoneurons survive long term compared with 47.2% (±4.4, S.E.M.; n=4) in parvalbumin over-expressing mice. Surprisingly, this dramatic increase in motoneuron survival was not reflected in a significant improvement in muscle function, since 8 weeks after injury there was no improvement in either maximal twitch and tetanic force, or muscle weights. Thus, inducing spinal motoneurons to express parvalbumin protects a large proportion of motoneurons from injury-induced cell death, but this is not sufficient to restore muscle function.