Bacterial ferritin contains 24 haem groups

Pseudomonas aeruginosa bacterioferritin, also known as cytochrome b 1 or cytochrome b 557, has been isolated with 9 haems per 24 subunits. Various forms of the protein have been prepared including the completely haem-free protein and the fully haem-loaded protein with 24 haems per 24 subunits. The p...

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Bibliographic Details
Published in:FEBS letters 1990-10, Vol.271 (1), p.141-143
Main Authors: Kadir, Fahmi H.A., Moore, Geoffrey R.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Bacterial ferritin
Bacterial Proteins
Biological and medical sciences
Cytochrome b 1
Cytochrome b Group - analysis
Cytochrome b1
Ferritins - analysis
Fundamental and applied biological sciences. Psychology
Haem content
Heme - analysis
Kinetics
Proteins
Pseudomonas aeruginosa
Pseudomonas aeruginosa - enzymology
Spectrophotometry, Ultraviolet
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Summary:Pseudomonas aeruginosa bacterioferritin, also known as cytochrome b 1 or cytochrome b 557, has been isolated with 9 haems per 24 subunits. Various forms of the protein have been prepared including the completely haem-free protein and the fully haem-loaded protein with 24 haems per 24 subunits. The presence of the core does not significantly affect haem addition or removal. The absorbance ratio of the non-haem-iron-loaded protein, 278 nm:417 nm (oxidised), can be used to estimate the haem loading.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)80391-U