Codon reading properties of an unmodified transfer RNA

We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the cou...

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Bibliographic Details
Published in:FEBS letters 1990-10, Vol.273 (1-2), p.173-176
Main Authors: Claesson, Catharina, Samuelsson, Tore, Lustig, Florentyna, Borén, Thomas
Format: Article
Language:English
Subjects:
Anticodon
Base Sequence
Biological and medical sciences
Capsid - biosynthesis
Capsid - genetics
Capsid - isolation & purification
Codon
Codon - genetics
DNA-Directed RNA Polymerases - metabolism
Fundamental and applied biological sciences. Psychology
Glycine-tRNA Ligase - metabolism
Modified nucleoside
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mycoplasma
Mycoplasma mycoides
Mycoplasma mycoides - genetics
Plasmids
Protein Biosynthesis
RNA, Transfer, Gly - genetics
Transcription, Genetic
tRNA
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Summary:We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investigation T7 RNA polymerase transcription of the corresponding gene was used to produce a tRNA devoid of modified nucleosides. Using an in vitro translation system the ability of this tRNA to read the 4 glycine codons (GGU, GGC, GGA and GGG) was tested and it was shown to be as efficient as its normal, fully modified counterpart in the reading of all four codons. This result demonstrates that a tRNA devoid of modified nucleosides is able to efficiently sustain protein synthesis in vitro and, furthermore, that the normal modification pattern of the Mycoplasma glycine tRNA is not essential for the ability of this tRNA to read the glycine codons GGU and GGC effectively.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)81077-2