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A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin
A novel multi-channel 2×5 model of piezoelectric quartz micro-array immunosensor has been developed for quantitative detection of human chorionic gonadotropin (hCG) in serum or urine samples. Every crystal unit of the fabricated piezoelectric hCG micro-array immunosensor can oscillate independently...
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| Published in: | Biosensors & bioelectronics 2004-02, Vol.19 (7), p.711-720 |
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| Main Authors: | , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c422t-8192008b10b95fb1c4dd958f17aa13d6538a19f53c6d7f500f9434bf45edec933 |
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| cites | cdi_FETCH-LOGICAL-c422t-8192008b10b95fb1c4dd958f17aa13d6538a19f53c6d7f500f9434bf45edec933 |
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| container_title | Biosensors & bioelectronics |
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| creator | Zhang, Bo Mao, Qiongguo Zhang, Xue Jiang, Tianlun Chen, Ming Yu, Fan Fu, Weiling |
| description | A novel multi-channel 2×5 model of piezoelectric quartz micro-array immunosensor has been developed for quantitative detection of human chorionic gonadotropin (hCG) in serum or urine samples. Every crystal unit of the fabricated piezoelectric hCG micro-array immunosensor can oscillate independently without interfering each other. A 2×5 model of micro-array immunosensor as compared with a one-channel immunosensor can provide eight times higher detection speeds for hCG assay. The anti-hCG antibody is deposited on the gold electrode’s surface of 10
MHz quartz AT-cut crystal by self-assembled technique using sulfosuccinimidyl 6-[3′-(2-pyridyldithio) propionamido] hexanoate (Sulfo-LC-SPDP), and serves as an antibody recognizing layer. The highly ordered self-assembled monolayers (SAM) ensure well-controlled surface structure and offer many advantages to the performance of the sensor. Compared with conventional antibody immobilization methods, the amount and the reaction activity of antibody monolayer coated by the SAM binding are bigger than those by the SPA method, and less non-specific binding caused by other analytes in sample is found. Under the optimized experimental conditions, the results showed that micro-array immunosensor quantitatively detected serum or urine hCG in the range of 2.5–500
mIU/ml with high precision (CV |
| doi_str_mv | 10.1016/S0956-5663(03)00260-4 |
| format | article |
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MHz quartz AT-cut crystal by self-assembled technique using sulfosuccinimidyl 6-[3′-(2-pyridyldithio) propionamido] hexanoate (Sulfo-LC-SPDP), and serves as an antibody recognizing layer. The highly ordered self-assembled monolayers (SAM) ensure well-controlled surface structure and offer many advantages to the performance of the sensor. Compared with conventional antibody immobilization methods, the amount and the reaction activity of antibody monolayer coated by the SAM binding are bigger than those by the SPA method, and less non-specific binding caused by other analytes in sample is found. Under the optimized experimental conditions, the results showed that micro-array immunosensor quantitatively detected serum or urine hCG in the range of 2.5–500
mIU/ml with high precision (CV<5%); other hormones in human serum and urine did not interfere with the determination markedly. Serum and urine samples of 60 patients were detected by the micro-array immunosensor, and the results agreed well with those given by the commercial radioimmunoassay test kit, with correlation coefficient of 0.92. After regeneration with urea solution the coated immunosensor can be reused five times without appreciable loss of activity.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(03)00260-4</identifier><identifier>PMID: 14709389</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Chorionic Gonadotropin - blood ; Chorionic Gonadotropin - urine ; Coated Materials, Biocompatible - chemical synthesis ; Coated Materials, Biocompatible - chemistry ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Human chorionic gonadotropin (hCG) ; Humans ; Immunoassay - instrumentation ; Immunoassay - methods ; Immunosensor ; Methods. Procedures. Technologies ; Piezoelectric quartz crystal ; Protein Array Analysis - instrumentation ; Protein Array Analysis - methods ; Quartz ; Reproducibility of Results ; Self-assembled monolayer (SAM) ; Sensitivity and Specificity ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2004-02, Vol.19 (7), p.711-720</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-8192008b10b95fb1c4dd958f17aa13d6538a19f53c6d7f500f9434bf45edec933</citedby><cites>FETCH-LOGICAL-c422t-8192008b10b95fb1c4dd958f17aa13d6538a19f53c6d7f500f9434bf45edec933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15474779$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14709389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Bo</creatorcontrib><creatorcontrib>Mao, Qiongguo</creatorcontrib><creatorcontrib>Zhang, Xue</creatorcontrib><creatorcontrib>Jiang, Tianlun</creatorcontrib><creatorcontrib>Chen, Ming</creatorcontrib><creatorcontrib>Yu, Fan</creatorcontrib><creatorcontrib>Fu, Weiling</creatorcontrib><title>A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A novel multi-channel 2×5 model of piezoelectric quartz micro-array immunosensor has been developed for quantitative detection of human chorionic gonadotropin (hCG) in serum or urine samples. Every crystal unit of the fabricated piezoelectric hCG micro-array immunosensor can oscillate independently without interfering each other. A 2×5 model of micro-array immunosensor as compared with a one-channel immunosensor can provide eight times higher detection speeds for hCG assay. The anti-hCG antibody is deposited on the gold electrode’s surface of 10
MHz quartz AT-cut crystal by self-assembled technique using sulfosuccinimidyl 6-[3′-(2-pyridyldithio) propionamido] hexanoate (Sulfo-LC-SPDP), and serves as an antibody recognizing layer. The highly ordered self-assembled monolayers (SAM) ensure well-controlled surface structure and offer many advantages to the performance of the sensor. Compared with conventional antibody immobilization methods, the amount and the reaction activity of antibody monolayer coated by the SAM binding are bigger than those by the SPA method, and less non-specific binding caused by other analytes in sample is found. Under the optimized experimental conditions, the results showed that micro-array immunosensor quantitatively detected serum or urine hCG in the range of 2.5–500
mIU/ml with high precision (CV<5%); other hormones in human serum and urine did not interfere with the determination markedly. Serum and urine samples of 60 patients were detected by the micro-array immunosensor, and the results agreed well with those given by the commercial radioimmunoassay test kit, with correlation coefficient of 0.92. After regeneration with urea solution the coated immunosensor can be reused five times without appreciable loss of activity.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chorionic Gonadotropin - blood</subject><subject>Chorionic Gonadotropin - urine</subject><subject>Coated Materials, Biocompatible - chemical synthesis</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human chorionic gonadotropin (hCG)</subject><subject>Humans</subject><subject>Immunoassay - instrumentation</subject><subject>Immunoassay - methods</subject><subject>Immunosensor</subject><subject>Methods. Procedures. Technologies</subject><subject>Piezoelectric quartz crystal</subject><subject>Protein Array Analysis - instrumentation</subject><subject>Protein Array Analysis - methods</subject><subject>Quartz</subject><subject>Reproducibility of Results</subject><subject>Self-assembled monolayer (SAM)</subject><subject>Sensitivity and Specificity</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkc1qFTEUgIMo9rb6CEo2ii5Gk0kyM1mVUqwKBRfqOmSSExvJz20yU7h9A9_aXO_FLgs5BA7f-eF8CL2i5AMldPj4nUgxdGIY2DvC3hPSD6TjT9CGTiPreM_EU7T5j5yg01p_E0JGKslzdEL5SCSb5Ab9ucAp30HAWw_3GQKYpXiDb1ddlnscvSm506XoHfYxrilXSDUXPOsKFueEKwTX6VohzqFlYk456B0U7BplYYESfdKLb2h2-GaNOmFzk0tLtDG_ctI2LyVvfXqBnjkdKrw8_mfo59WnH5dfuutvn79eXlx3hvf90k1U9oRMMyWzFG6mhlsrxeToqDVldhBs0lQ6wcxgRycIcZIzPjsuwIKRjJ2ht4e-25JvV6iLir4aCEEnyGtVU-vOaIvHwLZIzzntGygOYDtWrQWc2hYfddkpStRelvonS-1NKNLeXpbire71ccA6R7APVUc7DXhzBHQ1Oriik_H1gRN85OO4584PHLS73XkoqhoPyYD1pQlVNvtHVvkLtc20KQ</recordid><startdate>20040215</startdate><enddate>20040215</enddate><creator>Zhang, Bo</creator><creator>Mao, Qiongguo</creator><creator>Zhang, Xue</creator><creator>Jiang, Tianlun</creator><creator>Chen, Ming</creator><creator>Yu, Fan</creator><creator>Fu, Weiling</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040215</creationdate><title>A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin</title><author>Zhang, Bo ; Mao, Qiongguo ; Zhang, Xue ; Jiang, Tianlun ; Chen, Ming ; Yu, Fan ; Fu, Weiling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-8192008b10b95fb1c4dd958f17aa13d6538a19f53c6d7f500f9434bf45edec933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chorionic Gonadotropin - blood</topic><topic>Chorionic Gonadotropin - urine</topic><topic>Coated Materials, Biocompatible - chemical synthesis</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human chorionic gonadotropin (hCG)</topic><topic>Humans</topic><topic>Immunoassay - instrumentation</topic><topic>Immunoassay - methods</topic><topic>Immunosensor</topic><topic>Methods. Procedures. Technologies</topic><topic>Piezoelectric quartz crystal</topic><topic>Protein Array Analysis - instrumentation</topic><topic>Protein Array Analysis - methods</topic><topic>Quartz</topic><topic>Reproducibility of Results</topic><topic>Self-assembled monolayer (SAM)</topic><topic>Sensitivity and Specificity</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Bo</creatorcontrib><creatorcontrib>Mao, Qiongguo</creatorcontrib><creatorcontrib>Zhang, Xue</creatorcontrib><creatorcontrib>Jiang, Tianlun</creatorcontrib><creatorcontrib>Chen, Ming</creatorcontrib><creatorcontrib>Yu, Fan</creatorcontrib><creatorcontrib>Fu, Weiling</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Bo</au><au>Mao, Qiongguo</au><au>Zhang, Xue</au><au>Jiang, Tianlun</au><au>Chen, Ming</au><au>Yu, Fan</au><au>Fu, Weiling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2004-02-15</date><risdate>2004</risdate><volume>19</volume><issue>7</issue><spage>711</spage><epage>720</epage><pages>711-720</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A novel multi-channel 2×5 model of piezoelectric quartz micro-array immunosensor has been developed for quantitative detection of human chorionic gonadotropin (hCG) in serum or urine samples. Every crystal unit of the fabricated piezoelectric hCG micro-array immunosensor can oscillate independently without interfering each other. A 2×5 model of micro-array immunosensor as compared with a one-channel immunosensor can provide eight times higher detection speeds for hCG assay. The anti-hCG antibody is deposited on the gold electrode’s surface of 10
MHz quartz AT-cut crystal by self-assembled technique using sulfosuccinimidyl 6-[3′-(2-pyridyldithio) propionamido] hexanoate (Sulfo-LC-SPDP), and serves as an antibody recognizing layer. The highly ordered self-assembled monolayers (SAM) ensure well-controlled surface structure and offer many advantages to the performance of the sensor. Compared with conventional antibody immobilization methods, the amount and the reaction activity of antibody monolayer coated by the SAM binding are bigger than those by the SPA method, and less non-specific binding caused by other analytes in sample is found. Under the optimized experimental conditions, the results showed that micro-array immunosensor quantitatively detected serum or urine hCG in the range of 2.5–500
mIU/ml with high precision (CV<5%); other hormones in human serum and urine did not interfere with the determination markedly. Serum and urine samples of 60 patients were detected by the micro-array immunosensor, and the results agreed well with those given by the commercial radioimmunoassay test kit, with correlation coefficient of 0.92. After regeneration with urea solution the coated immunosensor can be reused five times without appreciable loss of activity.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>14709389</pmid><doi>10.1016/S0956-5663(03)00260-4</doi><tpages>10</tpages></addata></record> |
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| ispartof | Biosensors & bioelectronics, 2004-02, Vol.19 (7), p.711-720 |
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| source | Elsevier |
| subjects | Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Chorionic Gonadotropin - blood Chorionic Gonadotropin - urine Coated Materials, Biocompatible - chemical synthesis Coated Materials, Biocompatible - chemistry Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Human chorionic gonadotropin (hCG) Humans Immunoassay - instrumentation Immunoassay - methods Immunosensor Methods. Procedures. Technologies Piezoelectric quartz crystal Protein Array Analysis - instrumentation Protein Array Analysis - methods Quartz Reproducibility of Results Self-assembled monolayer (SAM) Sensitivity and Specificity Various methods and equipments |
| title | A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin |
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