Characterization of glucose uptake by Trichuris globulosa (Nematoda) in vitro

14C-glucose uptake by adult Trichuris globulosa is found to be a non-linear function of time and limiting substrate concentration. The uptake is a two component process, an initial rapid burst is followed by a lower steady state, implying a mediated process. The uptake is dependent on Na+ ions which...

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Bibliographic Details
Published in:Journal of helminthology 1990-09, Vol.64 (3), p.248-254
Main Authors: Jasra, Nisha, Sanyal, Sankar N., Khera, S.
Format: Article
Language:English
Subjects:
Absorption
Adenosine Monophosphate - pharmacology
Adenosine Triphosphate - pharmacology
Animals
Binding, Competitive
fenbendazole
glucose
Glucose - metabolism
Glucose - pharmacology
glucose uptake
goats
Hydrogen-Ion Concentration
inhibitors
intestines
Iodoacetates - pharmacology
Iodoacetic Acid
Kinetics
Maltose - pharmacology
nematode control
nutrient uptake
Ouabain - pharmacology
Phlorhizin - pharmacology
thiabendazole
Trichuris
Trichuris - metabolism
Trichuris globulosa
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Summary:14C-glucose uptake by adult Trichuris globulosa is found to be a non-linear function of time and limiting substrate concentration. The uptake is a two component process, an initial rapid burst is followed by a lower steady state, implying a mediated process. The uptake is dependent on Na+ ions which cannot be replaced by K+, Li+ or choline. The uptake is also dependent on pH, being maximal at pH 7·4. 14C-glucose absorption is markedly inhibited by glucose, phlorizin, ouabain and to a smaller extent by a number of monosaccharides and other sugar-phosphates, nucleosides and metabolic inhibitors like p-nitrophenyl phosphate and iodoacetate. The inhibition constant for glucose, phlorizin and ouabain has been found to be 8 mM, 5 mM and 7 mM, respectively. A modified Dixon-plot shows that glucose is a completely competitive inhibitor for 14C-glucose uptake while the nature of competitive inhibition of phlorizin and ouabain are found to be partial.
ISSN:0022-149X
1475-2697
DOI:10.1017/S0022149X00012232