Construction of high-density bacterial colony arrays and patterns by the ink-jet method

We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink‐jet printers. Bacterial colony arrays with a density of 100 colonies/cm2 were obtained by directly ejecting Escherichia coli (E. coli) onto agar‐coated substrates at a rapid array...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2004-01, Vol.85 (1), p.29-33
Main Authors: Xu, Tao, Petridou, Sevastioni, Lee, Eric H., Roth, Elizabeth A., Vyavahare, Narendra R., Hickman, James J., Boland, Thomas
Format: Article
Language:English
Subjects:
bacterial colony array
Biological and medical sciences
Biological Assay - instrumentation
Biological Assay - methods
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
biosensor
Biotechnology
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
cell density gradient
Cell Division - physiology
Colony Count, Microbial - instrumentation
Colony Count, Microbial - methods
complex pattern
Equipment Design
Equipment Failure Analysis
Escherichia coli - cytology
Escherichia coli - growth & development
Fundamental and applied biological sciences. Psychology
ink-jet printer
Methods. Procedures. Technologies
Others
Printing - instrumentation
Printing - methods
Robotics - instrumentation
Robotics - methods
Various methods and equipments
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Summary:We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink‐jet printers. Bacterial colony arrays with a density of 100 colonies/cm2 were obtained by directly ejecting Escherichia coli (E. coli) onto agar‐coated substrates at a rapid arraying speed of 880 spots per second. Adjusting the concentration of bacterial suspensions allowed single colonies of viable bacteria to be obtained. In addition, complex patterns of viable bacteria as well as bacteria density gradients were constructed using desktop printers controlled by a simple software program. © 2003 Wiley Periodicals, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.10768