Shifting Foci of Hematopoiesis during Reconstitution from Single Stem Cells

To reveal the early events and dynamics of hematopoietic reconstitution in living animals in real-time, we used bioluminescence imaging to monitor engraftment from single luciferase-labeled hematopoietic stem cells (HSC) in irradiated recipients. Transplanted HSC generated discrete foci in the splee...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2004-01, Vol.101 (1), p.221-226
Main Authors: Cao, Yu-An, Wagers, Amy J., Beilhack, Andreas, Dusich, Joan, Bachmann, Michael H., Negrin, Robert S., Weissman, Irving L., Contag, Christopher H.
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Bone marrow
Bone Marrow Cells - cytology
Chimera
Chimerism
Graft Survival
Green Fluorescent Proteins
Hematopoiesis
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells - cytology
Homing
Imaging
Luciferases - genetics
Luminescent Measurements
Luminescent Proteins - genetics
Mice
Mice, Transgenic
Photons
Recombinant Proteins - genetics
Spleen
Stem cells
Studies
T lymphocytes
Tissue transplantation
Transplantation
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Summary:To reveal the early events and dynamics of hematopoietic reconstitution in living animals in real-time, we used bioluminescence imaging to monitor engraftment from single luciferase-labeled hematopoietic stem cells (HSC) in irradiated recipients. Transplanted HSC generated discrete foci in the spleen and bone marrow (BM), at a frequency that correlated with BM compartment size. Initially detected foci could expand locally, seed other sites in BM or spleen, and/or recede with different kinetics. These studies reveal dynamic and variable patterns of engraftment from highly purified HSC and indicate that the final overall contribution of individual HSC to hematopoietic chimerism does not depend on the specific site of initial engraftment and expansion.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2637010100