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Achacin induces cell death in HeLa cells through two different mechanisms
Achacin, which belongs to the l-amino acid oxidase group, oxidizes free amino acids and produces hydrogen peroxide in cell culture systems. Morphological changes in cells incubated with achacin were similar to those of cells incubated with H 2O 2. In both cases, the end result was cell death. To exa...
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| Published in: | Archives of biochemistry and biophysics 2004-02, Vol.422 (1), p.103-109 |
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| Main Authors: | , , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c415t-677bec2c80de9be72b5d2a4daae7c1becace42d9e06a5685d49da4bf3403d7393 |
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| cites | cdi_FETCH-LOGICAL-c415t-677bec2c80de9be72b5d2a4daae7c1becace42d9e06a5685d49da4bf3403d7393 |
| container_end_page | 109 |
| container_issue | 1 |
| container_start_page | 103 |
| container_title | Archives of biochemistry and biophysics |
| container_volume | 422 |
| creator | Kanzawa, Nobuyuki Shintani, Satoko Ohta, Kazumasa Kitajima, Seiji Ehara, Tatsuya Kobayashi, Hiroko Kizaki, Harutoshi Tsuchiya, Takahide |
| description | Achacin, which belongs to the
l-amino acid oxidase group, oxidizes free amino acids and produces hydrogen peroxide in cell culture systems. Morphological changes in cells incubated with achacin were similar to those of cells incubated with H
2O
2. In both cases, the end result was cell death. To examine the mechanism of achacin-associated cytotoxicity, the H
2O
2 scavenger catalase was added to culture media. Features typical of apoptosis, including morphological changes, DNA fragmentation, and PARP cleavage, were observed when cells were incubated with achacin in the presence of catalase. Moreover, apoptosis was inhibited by Z–VAD–fmk, a broad-spectrum caspase inhibitor. Herein, we present evidence that two pathways are involved in achacin-induced cell death. One is direct generation of H
2O
2 through the
l-amino acid oxidase activity of achacin. The other is the caspase-mediated apoptotic pathway that is induced by depletion of
l-amino acids by achacin. |
| doi_str_mv | 10.1016/j.abb.2003.12.007 |
| format | article |
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l-amino acid oxidase group, oxidizes free amino acids and produces hydrogen peroxide in cell culture systems. Morphological changes in cells incubated with achacin were similar to those of cells incubated with H
2O
2. In both cases, the end result was cell death. To examine the mechanism of achacin-associated cytotoxicity, the H
2O
2 scavenger catalase was added to culture media. Features typical of apoptosis, including morphological changes, DNA fragmentation, and PARP cleavage, were observed when cells were incubated with achacin in the presence of catalase. Moreover, apoptosis was inhibited by Z–VAD–fmk, a broad-spectrum caspase inhibitor. Herein, we present evidence that two pathways are involved in achacin-induced cell death. One is direct generation of H
2O
2 through the
l-amino acid oxidase activity of achacin. The other is the caspase-mediated apoptotic pathway that is induced by depletion of
l-amino acids by achacin.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/j.abb.2003.12.007</identifier><identifier>PMID: 14725862</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Achacin ; Amino Acid Chloromethyl Ketones - pharmacology ; Amino Acid Oxidoreductases - metabolism ; Amino Acids - analysis ; Amino Acids - chemistry ; Amino Acids - deficiency ; Amino Acids - metabolism ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - physiology ; Caspase Inhibitors ; Caspases - metabolism ; Cell Survival - drug effects ; Cell Survival - physiology ; Chromosomes - drug effects ; Cysteine Proteinase Inhibitors - pharmacology ; DNA Fragmentation - drug effects ; DNA Fragmentation - physiology ; HeLa Cells ; Humans ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; L-Amino Acid Oxidase ; Neuropeptides - metabolism ; Neuropeptides - pharmacology ; Oxidation-Reduction</subject><ispartof>Archives of biochemistry and biophysics, 2004-02, Vol.422 (1), p.103-109</ispartof><rights>2003 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-677bec2c80de9be72b5d2a4daae7c1becace42d9e06a5685d49da4bf3403d7393</citedby><cites>FETCH-LOGICAL-c415t-677bec2c80de9be72b5d2a4daae7c1becace42d9e06a5685d49da4bf3403d7393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27900,27901</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14725862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kanzawa, Nobuyuki</creatorcontrib><creatorcontrib>Shintani, Satoko</creatorcontrib><creatorcontrib>Ohta, Kazumasa</creatorcontrib><creatorcontrib>Kitajima, Seiji</creatorcontrib><creatorcontrib>Ehara, Tatsuya</creatorcontrib><creatorcontrib>Kobayashi, Hiroko</creatorcontrib><creatorcontrib>Kizaki, Harutoshi</creatorcontrib><creatorcontrib>Tsuchiya, Takahide</creatorcontrib><title>Achacin induces cell death in HeLa cells through two different mechanisms</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Achacin, which belongs to the
l-amino acid oxidase group, oxidizes free amino acids and produces hydrogen peroxide in cell culture systems. Morphological changes in cells incubated with achacin were similar to those of cells incubated with H
2O
2. In both cases, the end result was cell death. To examine the mechanism of achacin-associated cytotoxicity, the H
2O
2 scavenger catalase was added to culture media. Features typical of apoptosis, including morphological changes, DNA fragmentation, and PARP cleavage, were observed when cells were incubated with achacin in the presence of catalase. Moreover, apoptosis was inhibited by Z–VAD–fmk, a broad-spectrum caspase inhibitor. Herein, we present evidence that two pathways are involved in achacin-induced cell death. One is direct generation of H
2O
2 through the
l-amino acid oxidase activity of achacin. The other is the caspase-mediated apoptotic pathway that is induced by depletion of
l-amino acids by achacin.</description><subject>Achacin</subject><subject>Amino Acid Chloromethyl Ketones - pharmacology</subject><subject>Amino Acid Oxidoreductases - metabolism</subject><subject>Amino Acids - analysis</subject><subject>Amino Acids - chemistry</subject><subject>Amino Acids - deficiency</subject><subject>Amino Acids - metabolism</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - physiology</subject><subject>Caspase Inhibitors</subject><subject>Caspases - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - physiology</subject><subject>Chromosomes - drug effects</subject><subject>Cysteine Proteinase Inhibitors - pharmacology</subject><subject>DNA Fragmentation - drug effects</subject><subject>DNA Fragmentation - physiology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>L-Amino Acid Oxidase</subject><subject>Neuropeptides - metabolism</subject><subject>Neuropeptides - pharmacology</subject><subject>Oxidation-Reduction</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kDFPwzAQhS0EoqXwA1hQJraEs-PYiZiqCihSJRaYLce-UFdNUuwExL_HpZXYmE56997T3UfINYWMAhV3m0zXdcYA8oyyDECekCmFSqSQl_yUTCFu0qoUdEIuQtgAUMoFOycTyiUrSsGm5Hlu1tq4LnGdHQ2GxOB2m1jUwzpKyRJX-lcKybD2_fi-ToavPrGuadBjNyQtxnznQhsuyVmjtwGvjnNG3h4fXhfLdPXy9LyYr1LDaTGkQsoaDTMlWKxqlKwuLNPcao3S0LjSBjmzFYLQhSgLyyured3kHHIr8yqfkdtD7873HyOGQbUu7E_UHfZjUCVAJatcRCM9GI3vQ_DYqJ13rfbfioLa81MbFfmpPT9FmYr8YubmWD7WLdq_xBFYNNwfDBhf_HToVTAOO4PWeTSDsr37p_4H4eSA5A</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Kanzawa, Nobuyuki</creator><creator>Shintani, Satoko</creator><creator>Ohta, Kazumasa</creator><creator>Kitajima, Seiji</creator><creator>Ehara, Tatsuya</creator><creator>Kobayashi, Hiroko</creator><creator>Kizaki, Harutoshi</creator><creator>Tsuchiya, Takahide</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040201</creationdate><title>Achacin induces cell death in HeLa cells through two different mechanisms</title><author>Kanzawa, Nobuyuki ; 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l-amino acid oxidase group, oxidizes free amino acids and produces hydrogen peroxide in cell culture systems. Morphological changes in cells incubated with achacin were similar to those of cells incubated with H
2O
2. In both cases, the end result was cell death. To examine the mechanism of achacin-associated cytotoxicity, the H
2O
2 scavenger catalase was added to culture media. Features typical of apoptosis, including morphological changes, DNA fragmentation, and PARP cleavage, were observed when cells were incubated with achacin in the presence of catalase. Moreover, apoptosis was inhibited by Z–VAD–fmk, a broad-spectrum caspase inhibitor. Herein, we present evidence that two pathways are involved in achacin-induced cell death. One is direct generation of H
2O
2 through the
l-amino acid oxidase activity of achacin. The other is the caspase-mediated apoptotic pathway that is induced by depletion of
l-amino acids by achacin.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>14725862</pmid><doi>10.1016/j.abb.2003.12.007</doi><tpages>7</tpages></addata></record> |
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| ispartof | Archives of biochemistry and biophysics, 2004-02, Vol.422 (1), p.103-109 |
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| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Achacin Amino Acid Chloromethyl Ketones - pharmacology Amino Acid Oxidoreductases - metabolism Amino Acids - analysis Amino Acids - chemistry Amino Acids - deficiency Amino Acids - metabolism Apoptosis Apoptosis - drug effects Apoptosis - physiology Caspase Inhibitors Caspases - metabolism Cell Survival - drug effects Cell Survival - physiology Chromosomes - drug effects Cysteine Proteinase Inhibitors - pharmacology DNA Fragmentation - drug effects DNA Fragmentation - physiology HeLa Cells Humans Hydrogen peroxide Hydrogen Peroxide - pharmacology L-Amino Acid Oxidase Neuropeptides - metabolism Neuropeptides - pharmacology Oxidation-Reduction |
| title | Achacin induces cell death in HeLa cells through two different mechanisms |
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