Proteome analysis of cultivar-specific deregulations of Oryza sativa indica and O. sativa japonica cellular suspensions undergoing Rice yellow mottle virus infection

We have used two‐dimensional gel electrophoresis with mass spectrometry analysis to study the temporal patterns of protein expression during RYMV (Rice yellow mottle virus) infection in rice cells of two cultivars: IR64, Oryza sativa indica, susceptible, and Azucena, O. sativa japonica, partially re...

Full description

Saved in:
Bibliographic Details
Published in:Proteomics (Weinheim) 2004-01, Vol.4 (1), p.216-225
Main Authors: Ventelon-Debout, Marjolaine, Delalande, François, Brizard, Jean-Paul, Diemer, Hélène, Van Dorsselaer, Alain, Brugidou, Christophe
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Heat-Shock Proteins - chemistry
Heat-Shock Proteins - physiology
Miscellaneous
Oryza - chemistry
Oryza - physiology
Oryza - virology
Oryza sativa
Plant Viruses - genetics
Plant Viruses - physiology
Protein identification
Proteins
Proteome - chemistry
Proteome - physiology
Rice yellow mottle virus
RNA - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have used two‐dimensional gel electrophoresis with mass spectrometry analysis to study the temporal patterns of protein expression during RYMV (Rice yellow mottle virus) infection in rice cells of two cultivars: IR64, Oryza sativa indica, susceptible, and Azucena, O. sativa japonica, partially resistant to RYMV. Proteomic analysis of nonstressed and RYMV inoculated cells showed statistically significant changes in the relative levels of 40 IR64 proteins and 24 Azucena proteins. Protein identification using mass spectrometry was attempted for all the differentially regulated proteins. This global analysis detected 32 hypothetical “new” proteins. Nineteen differentially regulated proteins were identified for IR64 cultivar, while 13 were identified for Azucena cultivar, including proteins in three functional categories: metabolism, stress‐related proteins, and translation. These data revealed that a number of proteins regulated by abiotic stress response pathway were activated by RYMV in both cultivars (such as salt‐induced protein, heat shock proteins (HSPs), superoxide dismutase (SOD), and others have functions consistent with the susceptibility or partially resistance trait (such as dehydrin, proteins involved in glycolysis pathway).
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200300502