A Cytoplasmic Protein Inhibits the GTPase Activity of H-Ras in a Phospholipid-Dependent Manner

A cytoplasmic protein has been identified that inhibits the guanosine triphosphatase (GTPase) activity of bacterially synthesized, cellular H-Ras protein. This GTPase inhibiting protein is able to counteract the activity of GTPase activating protein (GAP), which has been postulated to function as a...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1990-11, Vol.250 (4983), p.982-985
Main Authors: Tsai, Men-Hwei, Yu, Chun-Li, Stacey, Dennis W.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
brain
Cell Division
Cell proliferation
Cellular biology
Dose-Response Relationship, Drug
Enzymes and enzyme inhibitors
Fatty acids
Fundamental and applied biological sciences. Psychology
GTP Phosphohydrolases - antagonists & inhibitors
GTPase-Activating Proteins
GTPases
Guanosine triphosphatase
guanosinetriphosphatase
H-ras
Hydrolases
Hydrolysis
Lipids
Mice
Nucleotides
Phosphatidic acids
Phospholipids
Phospholipids - pharmacology
Physiological aspects
Proteins
Proteins - antagonists & inhibitors
Proto-Oncogene Proteins p21(ras) - antagonists & inhibitors
ras GTPase-Activating Proteins
Resins
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Summary:A cytoplasmic protein has been identified that inhibits the guanosine triphosphatase (GTPase) activity of bacterially synthesized, cellular H-Ras protein. This GTPase inhibiting protein is able to counteract the activity of GTPase activating protein (GAP), which has been postulated to function as a negative regulator of Ras activity. The potential biological importance of the GTPase inhibiting protein is further supported by its interaction with lipids. Phospholipids produced in cells as a consequence of mitogenic stimulation increase the activity of the GTPase inhibiting protein, as well as inhibit the activity of GAP. The interaction of such lipids with each of these two regulatory proteins would, therefore, tend to increase the biological activity of Ras and stimulate cell proliferation.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2237442