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PCR-RFLP to identify Leishmania ( Viannia) braziliensis and L. ( Leishmania) amazonensis causing American cutaneous leishmaniasis
A PCR-RFLP based method was developed to diagnose and identify the Leishmania species causing American cutaneous leishmaniasis (ACL) in a panel of clinical samples obtained from an endemic region of Brazil. The comparison of the results obtained by PCR-RFLP and PCR-hybridization in the identificatio...
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Published in: | Acta tropica 2004-03, Vol.90 (1), p.31-37 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | A PCR-RFLP based method was developed to diagnose and identify the
Leishmania species causing American cutaneous leishmaniasis (ACL) in a panel of clinical samples obtained from an endemic region of Brazil. The comparison of the results obtained by PCR-RFLP and PCR-hybridization in the identification of
Leishmania (
Viannia)
braziliensis and
L. (
Leishmania) amazonensis were highly concordant (
κ=91.5%). The PCR-RFLP method was reliable, fast and easy to conduct on biopsies and presents potential value of utmost importance for the diagnosis and identification of
Leishmania in clinical specimens, infected reservoirs and vectors. |
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ISSN: | 0001-706X 1873-6254 |
DOI: | 10.1016/j.actatropica.2003.10.008 |