Immobilization of erythropoietin to culture erythropoietin-dependent human leukemia cell line

To investigate the effect of immobilized cytokine, erythropoietin (Epo) was immobilized on a culture plate and the Epo-dependent human leukemia cell line UT-7/Epo then was cultured upon the plate. A photo-reactive gelatin was mixed with Epo and the mixture was cast on a plate. The plate was then irr...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2004-05, Vol.25 (12), p.2293-2298
Main Authors: Ito, Yoshihiro, Hasuda, Hirokazu, Yamauchi, Tetsuya, Komatsu, Norio, Ikebuchi, Kenji
Format: Article
Language:English
Subjects:
Adsorption
Apoptosis
Biocompatible Materials - chemical synthesis
Biocompatible Materials - radiation effects
Cell Adhesion
Cell Culture Techniques - methods
Cell Division
Cell Line, Tumor
Cytokine
Erythropoietin
Erythropoietin - chemistry
Erythropoietin - metabolism
Gelatin - chemistry
Gelatin - radiation effects
Humans
Immobilization
Leukemia - pathology
Leukemia - physiopathology
Materials Testing
Micropatterning
Photo-lithography
Photochemistry - methods
Protein Binding
Tissue Engineering - methods
Ultraviolet Rays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To investigate the effect of immobilized cytokine, erythropoietin (Epo) was immobilized on a culture plate and the Epo-dependent human leukemia cell line UT-7/Epo then was cultured upon the plate. A photo-reactive gelatin was mixed with Epo and the mixture was cast on a plate. The plate was then irradiated with ultraviolet light in the presence or absence of a photo-mask. After washing with water, a micropatterned or unpatterned surface was formed. A leukemia cell line dependent on Epo, UT-7/Epo, was cultured on the sample plate. On the micropatterned surface, apoptosis of cells was induced on the surface without Epo, but was not observed on the Epo-immobilized surface. This result demonstrated that Epo stimulated the cells even after immobilization. Although the activity of immobilized Epo was low, the activity was slightly higher than that achieved by soluble Epo at higher concentration. In addition, the immobilized Epo could be repeatedly used for culture of UT-7/Epo cell. The present study provided a convenient immobilization method and indicated that immobilization of cytokines will be useful for creating an artificial cell culture device.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2003.09.002