Does granulosa cell apoptosis have a role in the etiology of unexplained infertility?

Objective( s): To compare granulosa cell apoptosis in patients with unexplained infertility and tubal factor. Accelerated granulosa cell apoptosis may be the cause of unexplained infertility. Study design: Setting was IVF-ET Unit of Cerrahpasa Medical Faculty, İstanbul University. GnRH analogs and g...

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Bibliographic Details
Published in:European journal of obstetrics & gynecology and reproductive biology 2004-02, Vol.112 (2), p.182-184
Main Authors: İdil, Mehmet, Çepni, İsmail, Demirsoy, Gülten, Öcal, Pelin, Salihoğlu, Funda, Şenol, Hülya, Elibol, Feyhan, İrez, Tülay
Format: Article
Language:English
Subjects:
Adult
Apoptosis
Apoptosis - physiology
Biological and medical sciences
Birth control
Cells, Cultured
Embryo Transfer
Female
Fertilization in Vitro - methods
Follicle Stimulating Hormone - therapeutic use
Gonadotropin-Releasing Hormone - therapeutic use
Granulosa cell
Granulosa Cells - pathology
Gynecology. Andrology. Obstetrics
Humans
Infertility, Female - etiology
Infertility, Female - therapy
Medical sciences
Ovulation Induction - methods
Probability
Prospective Studies
Risk Factors
Sensitivity and Specificity
Statistics, Nonparametric
Sterility. Assisted procreation
Unexplained infertility
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Summary:Objective( s): To compare granulosa cell apoptosis in patients with unexplained infertility and tubal factor. Accelerated granulosa cell apoptosis may be the cause of unexplained infertility. Study design: Setting was IVF-ET Unit of Cerrahpasa Medical Faculty, İstanbul University. GnRH analogs and gonadotropins were used for ovulation induction in patients with unexplained infertility ( n=15) and tubal factor ( n=15) undergoing in vitro fertilization and embryo transfer (IVF-ET) procedures. Following HCG injection and follicular aspiration, apoptosis of granulosa cells was assessed using the in situ DNA nick end labelling method and apoptosis rate was further determined by flow cytometry. Apoptosis rates were compared between two groups. Mann–Whitney’s U-test and Student’s t-test were used for statistics. Results: Apoptosis rate was significantly higher in the unexplained infertility group (33.20±35.62% versus 10.10±17.23%). Conclusion( s): Granulosa cell apoptosis seems to have a role in the etiology of unexplained infertility.
ISSN:0301-2115
1872-7654
DOI:10.1016/S0301-2115(03)00365-8